Show simple item record

dc.contributor.authorGhosh, Anna K
dc.contributor.authorMoore, Michael
dc.contributor.authorStreet, A J
dc.contributor.authorHowat, J
dc.contributor.authorSchofield, Philip F
dc.date.accessioned2010-11-30T17:04:59Z
dc.date.available2010-11-30T17:04:59Z
dc.date.issued1986-10-15
dc.identifier.citationExpression of HLA-D sub-region products on human colorectal carcinoma. 1986, 38 (4):459-64 Int J Canceren
dc.identifier.issn0020-7136
dc.identifier.pmid2428757
dc.identifier.doi10.1002/ijc.2910380402
dc.identifier.urihttp://hdl.handle.net/10541/116770
dc.description.abstractThe major histocompatibility complex (MHC) status of normal, inflamed, pre-malignant and malignant epithelia of the human gastrointestinal tract was investigated by immunocytochemical methods using monoclonal antibodies (MAbs) directed against heavy (alpha)- and light (beta 2m)-chain Class-I molecules and sub-locus products (DP, DQ, DR) of the HLA-D region. Class-I expression on epithelial cells appeared to vary little with pathological status except in the case of 4/32 (13%) colorectal carcinomas in which the antigens were undetectable or scanty. The pattern of Class-II expression was more complex. The antigens were readily detectable on normal stomach epithelium, in villous adenomas and in inflammatory bowel mucosa. In each of these situations DR was the predominant specificity, followed by DP and DQ. Expression on normal colonic epithelium was usually negative but, in the vicinity of a neoplasm or an area of marked leukocyte infiltration, Class-II molecules (DR greater than DP much greater than DQ) were detectable. A similar pattern of non-coordinate expression was found on 23/32 (72%) colorectal carcinomas, but on the remaining 28% no Class-II products were detectable, under conditions wherein stromal leukocytes were strongly stained. The data suggest that in a significant proportion (nearly 30%) of primary colorectal carcinomas, the capacity for Class-II induction, a constitutive or acquired feature of normal colorectal epithelium, is either diminished or lost. Also, tumor Class-II status is not correlated to Dukes' stage or differentiation.
dc.language.isoenen
dc.subjectColonic Canceren
dc.subjectRectal Canceren
dc.subjectStomach Canceren
dc.subject.meshAdenoma
dc.subject.meshAged
dc.subject.meshAged, 80 and over
dc.subject.meshCarcinoma
dc.subject.meshColon
dc.subject.meshColonic Neoplasms
dc.subject.meshEpitopes
dc.subject.meshFemale
dc.subject.meshHLA-D Antigens
dc.subject.meshHLA-DP Antigens
dc.subject.meshHLA-DQ Antigens
dc.subject.meshHLA-DR Antigens
dc.subject.meshHistocompatibility Antigens
dc.subject.meshHistocytochemistry
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicroscopy, Electron
dc.subject.meshMiddle Aged
dc.subject.meshRectal Neoplasms
dc.subject.meshStomach Neoplasms
dc.titleExpression of HLA-D sub-region products on human colorectal carcinoma.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Laboratories, Christie Hospital and Holt Institute, Manchester M20 9BX, UK.en
dc.identifier.journalInternational Journal of Canceren
html.description.abstractThe major histocompatibility complex (MHC) status of normal, inflamed, pre-malignant and malignant epithelia of the human gastrointestinal tract was investigated by immunocytochemical methods using monoclonal antibodies (MAbs) directed against heavy (alpha)- and light (beta 2m)-chain Class-I molecules and sub-locus products (DP, DQ, DR) of the HLA-D region. Class-I expression on epithelial cells appeared to vary little with pathological status except in the case of 4/32 (13%) colorectal carcinomas in which the antigens were undetectable or scanty. The pattern of Class-II expression was more complex. The antigens were readily detectable on normal stomach epithelium, in villous adenomas and in inflammatory bowel mucosa. In each of these situations DR was the predominant specificity, followed by DP and DQ. Expression on normal colonic epithelium was usually negative but, in the vicinity of a neoplasm or an area of marked leukocyte infiltration, Class-II molecules (DR greater than DP much greater than DQ) were detectable. A similar pattern of non-coordinate expression was found on 23/32 (72%) colorectal carcinomas, but on the remaining 28% no Class-II products were detectable, under conditions wherein stromal leukocytes were strongly stained. The data suggest that in a significant proportion (nearly 30%) of primary colorectal carcinomas, the capacity for Class-II induction, a constitutive or acquired feature of normal colorectal epithelium, is either diminished or lost. Also, tumor Class-II status is not correlated to Dukes' stage or differentiation.


This item appears in the following Collection(s)

Show simple item record