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dc.contributor.authorAplin, J
dc.contributor.authorCampbell, S
dc.contributor.authorDonnai, P
dc.contributor.authorBard, J
dc.contributor.authorAllen, Terence D
dc.date.accessioned2010-11-30T17:47:54Z
dc.date.available2010-11-30T17:47:54Z
dc.date.issued1986-09
dc.identifier.citationImportance of vitamin C in maintenance of the normal amnion: an experimental study., 7 (5):377-89 Placentaen
dc.identifier.issn0143-4004
dc.identifier.pmid3786291
dc.identifier.doi10.1016/S0143-4004(86)80026-1
dc.identifier.urihttp://hdl.handle.net/10541/116741
dc.description.abstractCollagenous matrix in amnion accounts for most of the dry weight of the tissue and provides its mechanical strength and resistance to rupture. Cell and organ culture techniques have been utilized to study the influence of vitamin C upon the synthesis and deposition of extracellular matrix by cells of normal amnion at term. The cultures have been examined using light and electron microscopy and metabolic labelling. These studies show that both epithelial cells and fibroblasts of the deeper stromal layer are active in the production of fibrillar matrix at this time. Matrix deposition by epithelial cells in culture increases several-fold when the vitamin C concentration in the supernatant medium is increased from zero to 50 micrograms/ml, and, at the latter concentration, a continuous anastomosing fibrillar collagenous meshwork appears beneath the cells. This study thus provides new evidence that an adequate dietary supply of vitamin C is probably needed to maintain the strength of the chorioamnion.
dc.language.isoenen
dc.subjectFoetal Membranesen
dc.subject.meshAmnion
dc.subject.meshAscorbic Acid
dc.subject.meshCollagen
dc.subject.meshExtracellular Matrix
dc.subject.meshFemale
dc.subject.meshFetal Membranes, Premature Rupture
dc.subject.meshFibroblasts
dc.subject.meshHistocytochemistry
dc.subject.meshHumans
dc.subject.meshOrgan Culture Techniques
dc.subject.meshPregnancy
dc.titleImportance of vitamin C in maintenance of the normal amnion: an experimental study.en
dc.typeArticleen
dc.contributor.departmentDepartment of Obstetrics and Gynaecology, University of Manchester, St Mary's Hospital, Manchester M13 0FH, UKen
dc.identifier.journalPlacentaen
html.description.abstractCollagenous matrix in amnion accounts for most of the dry weight of the tissue and provides its mechanical strength and resistance to rupture. Cell and organ culture techniques have been utilized to study the influence of vitamin C upon the synthesis and deposition of extracellular matrix by cells of normal amnion at term. The cultures have been examined using light and electron microscopy and metabolic labelling. These studies show that both epithelial cells and fibroblasts of the deeper stromal layer are active in the production of fibrillar matrix at this time. Matrix deposition by epithelial cells in culture increases several-fold when the vitamin C concentration in the supernatant medium is increased from zero to 50 micrograms/ml, and, at the latter concentration, a continuous anastomosing fibrillar collagenous meshwork appears beneath the cells. This study thus provides new evidence that an adequate dietary supply of vitamin C is probably needed to maintain the strength of the chorioamnion.


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