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dc.contributor.authorMoore, James V
dc.contributor.authorKeene, J P
dc.contributor.authorLand, Edward J
dc.date.accessioned2010-11-30T17:32:15Z
dc.date.available2010-11-30T17:32:15Z
dc.date.issued1986-03
dc.identifier.citationDose-response relationships for photodynamic injury to murine skin. 1986, 59 (699):257-61 Br J Radiolen
dc.identifier.issn0007-1285
dc.identifier.pmid3947839
dc.identifier.doi10.1259/0007-1285-59-699-257
dc.identifier.urihttp://hdl.handle.net/10541/116739
dc.description.abstractOedema and necrosis of murine tail skin have been measured after intravenous injection of haematoporphyrin derivative ("Photofrin", or PhI) followed 24 h later by graded exposures of the tail to full-spectrum visible light from a quartz-halogen lamp. End-points were degree of oedema and the proportion of mice in a dose-group that developed skin necrosis and tail atrophy. Oedema developed within 24 h of illumination and was a function of PhI dose and duration of light exposure. Onset of necrosis occurred after a minimum of 5 days and onset time was an inverse function of exposure time. Probit plots of proportion of necroses versus light exposure yielded values for ND50 (exposure corresponding to 50% incidence of necrosis) and l/slope. At the high but non-toxic dose of 2 mg PhI/mouse, ND50 was 18 min, l/slope 4 min, for pigmented BDF1 mice. Halving the PhI dose increased ND50 by a factor of 1.9. Albino BALB/c mice were markedly more sensitive to 2 mg PhI plus light than BDF1 mice: the ND50 was 7 min. Temporary occlusion of the blood supply to the tail (10 min before and during illumination) abrogated totally the oedematous and necrotic reactions to photodynamic therapy.
dc.language.isoenen
dc.subjectHaematoporphyrin Photoradiationen
dc.subject.meshAnimals
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshEdema
dc.subject.meshHematoporphyrin Photoradiation
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred BALB C
dc.subject.meshMice, Inbred DBA
dc.subject.meshNecrosis
dc.subject.meshPhotochemotherapy
dc.subject.meshSkin
dc.subject.meshTime Factors
dc.titleDose-response relationships for photodynamic injury to murine skin.en
dc.typeArticleen
dc.contributor.departmentDepartments of Radiobiology, Physics and Instrumentation, and Biophysical Chemistry, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BXen
dc.identifier.journalThe British Journal of Radiologyen
html.description.abstractOedema and necrosis of murine tail skin have been measured after intravenous injection of haematoporphyrin derivative ("Photofrin", or PhI) followed 24 h later by graded exposures of the tail to full-spectrum visible light from a quartz-halogen lamp. End-points were degree of oedema and the proportion of mice in a dose-group that developed skin necrosis and tail atrophy. Oedema developed within 24 h of illumination and was a function of PhI dose and duration of light exposure. Onset of necrosis occurred after a minimum of 5 days and onset time was an inverse function of exposure time. Probit plots of proportion of necroses versus light exposure yielded values for ND50 (exposure corresponding to 50% incidence of necrosis) and l/slope. At the high but non-toxic dose of 2 mg PhI/mouse, ND50 was 18 min, l/slope 4 min, for pigmented BDF1 mice. Halving the PhI dose increased ND50 by a factor of 1.9. Albino BALB/c mice were markedly more sensitive to 2 mg PhI plus light than BDF1 mice: the ND50 was 7 min. Temporary occlusion of the blood supply to the tail (10 min before and during illumination) abrogated totally the oedematous and necrotic reactions to photodynamic therapy.


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