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dc.contributor.authorGhosh, Anna Ken
dc.contributor.authorMoore, Michaelen
dc.contributor.authorHarris, Martinen
dc.date.accessioned2010-11-30T16:51:47Z
dc.date.available2010-11-30T16:51:47Z
dc.date.issued1986-04
dc.identifier.citationImmunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man. 1986, 39 (4):428-34 J Clin Patholen
dc.identifier.issn0021-9746
dc.identifier.pmid3009557
dc.identifier.doi10.1136/jcp.39.4.428
dc.identifier.urihttp://hdl.handle.net/10541/116738
dc.description.abstractThe monoclonal antibody RAP-5 generated against a synthetic peptide corresponding to amino acid positions 10-17 of the ras p21 protein was used in an immunohistochemical study of the expression of ras in normal, benign, and malignant breast epithelium in man. The staining intensity and intracellular distribution of RAP-5 was similar in the three epithelial populations and extended to other tissue elements including myoepithelial cells, smooth muscle, myelin, capillary endothelium, and stromal fibroblasts, as well as sebaceous glands and sweat glands overlying the breast. These results suggest that RAP-5 recognises a normal cellular component, the expression of which is not more enhanced in hyperplastic or neoplastic conditions. The detection of mutant forms of p21 exclusively expressed in malignant tumours requires that alternative reagents be developed.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAdenofibroma
dc.subject.meshBreast
dc.subject.meshBreast Neoplasms
dc.subject.meshCarcinoma, Intraductal, Noninfiltrating
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshImmunoenzyme Techniques
dc.subject.meshOncogenes
dc.subject.meshProtein Biosynthesis
dc.subject.meshProto-Oncogene Proteins
dc.subject.meshProto-Oncogene Proteins p21(ras)
dc.titleImmunohistochemical detection of ras oncogene p21 product in benign and malignant mammary tissue in man.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester, M20 9BX, UK.en
dc.identifier.journalJournal of Clinical Pathologyen
html.description.abstractThe monoclonal antibody RAP-5 generated against a synthetic peptide corresponding to amino acid positions 10-17 of the ras p21 protein was used in an immunohistochemical study of the expression of ras in normal, benign, and malignant breast epithelium in man. The staining intensity and intracellular distribution of RAP-5 was similar in the three epithelial populations and extended to other tissue elements including myoepithelial cells, smooth muscle, myelin, capillary endothelium, and stromal fibroblasts, as well as sebaceous glands and sweat glands overlying the breast. These results suggest that RAP-5 recognises a normal cellular component, the expression of which is not more enhanced in hyperplastic or neoplastic conditions. The detection of mutant forms of p21 exclusively expressed in malignant tumours requires that alternative reagents be developed.


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