Molecular analysis of X-ray-induced mutants at the HPRT locus in V79 Chinese hamster cells.
Affiliation
Biomedical Sciences Division, Lawrence Livermore National Laboratory, University of California, Livermore, California, 94550, U.S.A.Issue Date
1986-06
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Spontaneous and X-ray-induced mutants at the hypoxanthine phosphoribosyl transferase (HPRT) locus have been isolated from V79 Chinese hamster cells and characterized at the biochemical and cytogenetic levels. Fourteen spontaneous and 24 X-ray-induced clones were azaguanine and thioguanine resistant, did not grow in HAT medium (AZRTGRHATS) and failed to incorporate significant levels of [14C]hypoxyanthine. Cytogenetic analysis of two spontaneous and eight X-ray-induced mutants revealed no major X chromosome rearrangements. In two induced mutants, one of which was hypotetraploid (mode 35-39) with 2 X chromosomes, the short arm of the chromosome (Xp) was slightly shorter than normal. A third mutant was hyperdiploid (mode 22-23) compared with the parental clone (mode 21). When compared with wild-type clones, no other cytogenetic changes were evident in the remaining mutants. Analysis at the DNA level using a Chinese hamster HPRT cDNA probe showed major deletion of HPRT sequences in two and partial deletion in another two induced mutants. In two of the mutants with deletions of HPRT sequences there was a visible shortening of the Xp arm. In the other six mutants two spontaneous and four induced) no karyotypic changes or alterations in restriction fragment patterns were detected suggesting that they carry small deletions or point mutations at the HPRT locus.Citation
Molecular analysis of X-ray-induced mutants at the HPRT locus in V79 Chinese hamster cells. 1986, 49 (6):1011-20 Int J Radiat Biol Relat Stud Phys Chem MedJournal
International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and MedicineDOI
10.1080/09553008514553231PubMed ID
3486844Type
ArticleLanguage
enISSN
0020-7616ae974a485f413a2113503eed53cd6c53
10.1080/09553008514553231