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    Reactions of reducing radicals with ribonuclease.

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    Authors
    Schuessler, H
    Davies, J V
    Scherbaum, W
    Jung, E
    Affiliation
    Institut für Radiologie der Universität Erlangen-Nürnberg, F.R.Germany
    Issue Date
    1986-11
    
    Metadata
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    Abstract
    Radiation-induced reactions of hydrated electrons, formate- and ethanol radicals with ribonuclease were studied by pulse radiolysis and by electrophoresis. Initially formate radicals react rapidly and very specifically with the disulphide bonds of ribonuclease. This reaction leads to aggregation by intermolecular S-S-interchange, the process being more effective at pH 4, since formation and decay of S-S-.-radical anions increases with decreasing pH. With high doses additional unreducible aggregates are formed. Radical formation at the positively charged histidine residues seems to be involved. Hydrated electrons do not react as selectively as the formate radicals, but with several sites in native ribonuclease. Thus with low doses unreducible aggregates are formed. Electrophoresis shows that reaction of the electrons causes fragmentation of the peptide chain, when OH-radicals are scavenged. Very weak transient spectra and very little degradation result on reaction of ethanol radicals with ribonuclease.
    Citation
    Reactions of reducing radicals with ribonuclease. 1986, 50 (5):825-39 Int J Radiat Biol Relat Stud Phys Chem Med
    Journal
    International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
    URI
    http://hdl.handle.net/10541/116031
    PubMed ID
    3490448
    Type
    Article
    Language
    en
    ISSN
    0020-7616
    Collections
    All Paterson Institute for Cancer Research

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