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dc.contributor.authorHartley, John A
dc.contributor.authorFox, Brian W
dc.date.accessioned2010-11-22T18:06:55Z
dc.date.available2010-11-22T18:06:55Z
dc.date.issued1986
dc.identifier.citationCross-linking between histones and DNA following treatment with a series of dimethane sulphonate esters. 1986, 17 (1):56-62 Cancer Chemother Pharmacolen
dc.identifier.issn0344-5704
dc.identifier.pmid3009042
dc.identifier.doi10.1007/BF00299867
dc.identifier.urihttp://hdl.handle.net/10541/116026
dc.description.abstractThe cross-linking of the nucleohistone to the associated DNA by a series of methanesulphonates of the busulphan series (CH3SO2O(CH2)nOSO2CH3) from n = 4-9 has been studied by gel electrophoresis, by the use of 35S labelling and by pyrenemaleimide competition. It has been shown that all the dimethanesulphonates react with the Cys 114 of histone H3, and that octamethylene dimethanesulphonate (n = 8) cross-links histone H3 with DNA via the sulphydryl group, as well as forming H3-H3 dimers.
dc.language.isoenen
dc.subjectAnticancerous Agentsen
dc.subjectCancer DNAen
dc.subject.meshAnimals
dc.subject.meshAntineoplastic Agents
dc.subject.meshCell Line
dc.subject.meshChromatin
dc.subject.meshChromosome Aberrations
dc.subject.meshCysteine
dc.subject.meshDNA, Neoplasm
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshHistones
dc.subject.meshMesylates
dc.subject.meshProtein Binding
dc.subject.meshRats
dc.subject.meshSarcoma, Yoshida
dc.subject.meshSpectrometry, Fluorescence
dc.titleCross-linking between histones and DNA following treatment with a series of dimethane sulphonate esters.en
dc.typeArticleen
dc.identifier.eissn1432-0843
dc.contributor.departmentPaterson Laboratories, Christie Hospital and Holt Radium Institute, Wilmslow Road, Withington, Manchester M20 9BX, UKen
dc.identifier.journalCancer Chemotherapy and Pharmacologyen
html.description.abstractThe cross-linking of the nucleohistone to the associated DNA by a series of methanesulphonates of the busulphan series (CH3SO2O(CH2)nOSO2CH3) from n = 4-9 has been studied by gel electrophoresis, by the use of 35S labelling and by pyrenemaleimide competition. It has been shown that all the dimethanesulphonates react with the Cys 114 of histone H3, and that octamethylene dimethanesulphonate (n = 8) cross-links histone H3 with DNA via the sulphydryl group, as well as forming H3-H3 dimers.


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