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    A structural basis for R- and T-banding: a scanning electron microscopy study.

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    Authors
    Jack, Elspeth M
    Harrison, Christine J
    Allen, Terence D
    Harris, R
    Affiliation
    Department of Ultrastructure Paterson Laboratories, Christie Hospital, Manchester M20 9BX UK
    Issue Date
    1986
    
    Metadata
    Show full item record
    Abstract
    The structure of reverse (R)-banded and telomeric (T)-banded chromosomes was studied by examination of the same chromosomes first in the light microscope (LM) followed by the scanning electron microscope (SEM). This procedure demonstrated a structural basis to both the R- and T-banding techniques. A direct correlation was shown between the LM staining patterns and the structural patterns observed in the SEM. In the R-banded chromosomes the positively stained R-bands, viewed by LM, corresponded to highly fibrous three-dimensional regions in the SEM. The negatively stained R-interbands corresponded to flatter regions from which material appeared to have been extracted. These structural observations strongly support the suggestion that chromosomal material is preferentially lost from the R-interbands with aggregation of fibres in the R-bands. T-banded chromosomes showed a similar structure to the R-banded chromosomes. The positively stained T-bands located at the telomeres corresponded to regions of highly aggregated fibres. The remainder of the chromosome, corresponding to the negatively stained area, had a flattened and extracted appearance. These similarities in morphology between the T- and R-banded chromosomes support the view that T-bands result from a progressive breakdown of the R-banded chromosome structure.
    Citation
    A structural basis for R- and T-banding: a scanning electron microscopy study. 1986, 94 (5):395-402 Chromosoma
    Journal
    Chromosoma
    URI
    http://hdl.handle.net/10541/116021
    DOI
    10.1007/BF00328640
    PubMed ID
    3829828
    Type
    Article
    Language
    en
    ISSN
    0009-5915
    EISSN
    1432-0886
    ae974a485f413a2113503eed53cd6c53
    10.1007/BF00328640
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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