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dc.contributor.authorSaffhill, Roy
dc.contributor.authorHume, W J
dc.date.accessioned2010-11-22T17:43:43Z
dc.date.available2010-11-22T17:43:43Z
dc.date.issued1986-03
dc.identifier.citationThe degradation of 5-iododeoxyuridine and 5-bromodeoxyuridine by serum from different sources and its consequences for the use of the compounds for incorporation into DNA. 1986, 57 (3):347-55 Chem Biol Interacten
dc.identifier.issn0009-2797
dc.identifier.pmid3698122
dc.identifier.doi10.1016/0009-2797(86)90008-6
dc.identifier.urihttp://hdl.handle.net/10541/115997
dc.description.abstractEnzymes are present in sera that convert 5-iododeoxyuridine (IdU) to deoxyuridine (dU) and 5-iodouracil (IU). Although in the presence of serum 5-bromodeoxyuridine (BrdU) is not subject to extensive debromination it is converted to 5-bromouracil (BrU) at approx. 50% of the rate for IdU. These conversions are likely brought about by the enzymes thymidylate synthetase and thymidine phosphorylase. In vivo and in culture the dU enters DNA as thymidine 5'-monophosphate (dTMP) via the de novo pathway. Deoxyuridine is often found as a contaminant of [3H]IdU and [3H]BrdU. For these reasons, complications can arise in the interpretation of experimental work using these radioactive compounds. The problems may be overcome by purifying the compounds by high performance liquid chromatography (HPLC) before use together with identification of the DNA components with which the 3H is associated by chromatographic analysis.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBromodeoxyuridine
dc.subject.meshBromouracil
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshDNA
dc.subject.meshDeoxyuridine
dc.subject.meshHorses
dc.subject.meshIdoxuridine
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshRabbits
dc.subject.meshSpecies Specificity
dc.subject.meshThyroid Gland
dc.subject.meshUracil
dc.titleThe degradation of 5-iododeoxyuridine and 5-bromodeoxyuridine by serum from different sources and its consequences for the use of the compounds for incorporation into DNA.en
dc.typeArticleen
dc.identifier.eissn1872-7786
dc.contributor.departmentPaterson Laboratories, Christie Hospital, Manchester M20 9BX UKen
dc.identifier.journalChemico-Biological Interactionsen
html.description.abstractEnzymes are present in sera that convert 5-iododeoxyuridine (IdU) to deoxyuridine (dU) and 5-iodouracil (IU). Although in the presence of serum 5-bromodeoxyuridine (BrdU) is not subject to extensive debromination it is converted to 5-bromouracil (BrU) at approx. 50% of the rate for IdU. These conversions are likely brought about by the enzymes thymidylate synthetase and thymidine phosphorylase. In vivo and in culture the dU enters DNA as thymidine 5'-monophosphate (dTMP) via the de novo pathway. Deoxyuridine is often found as a contaminant of [3H]IdU and [3H]BrdU. For these reasons, complications can arise in the interpretation of experimental work using these radioactive compounds. The problems may be overcome by purifying the compounds by high performance liquid chromatography (HPLC) before use together with identification of the DNA components with which the 3H is associated by chromatographic analysis.


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