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    Foetal and cancer patient fibroblasts produce an autocrine migration-stimulating factor not made by normal adult cells.

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    Authors
    Schor, Seth L
    Schor, Ana M
    Grey, A
    Rushton, Graham
    Affiliation
    Department of Cell and Structural Biology, University of Manchester, England.
    Issue Date
    1988-07
    
    Metadata
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    Abstract
    We have previously reported that (1) the migration of foetal and adult fibroblasts into three-dimensional collagen matrices is differentially affected by cell density, and (2) skin fibroblasts from cancer patients commonly display a foetal-like mode of migratory behaviour. Data presented here indicate that differences in the migration of these cell types are particularly apparent in cultures plated at high density (i.e. at cell confluence); under these conditions, foetal fibroblasts and the foetal-like fibroblasts of cancer patients migrate into the three-dimensional collagen matrix to a significantly greater extent than do normal adult cells. In this initial study concerned with the biochemical basis of these observations, we report that medium conditioned by either foetal or cancer patient fibroblasts stimulates the migration of confluent adult cells. This stimulation of migration is specific to confluent cells, as the migration of subconfluent adult fibroblasts is unaffected by these conditioned media. Gel filtration chromatography of foetal fibroblast-conditioned medium indicates that migration-stimulating activity is recovered in a single peak with an apparent molecular mass in the range of 50-60 (X 10(3]. The active migration stimulating factor (MSF) in both foetal and cancer patient fibroblast-conditioned media appears to be a protein stable at acid pH, but inactivated by heat, alkaline pH and reductive alkylation. MSF produced by foetal and cancer patient fibroblasts is presumably responsible for the characteristically elevated levels of migration displayed by these cells in confluent culture, thereby suggesting an autocrine mode of action for this factor.(ABSTRACT TRUNCATED AT 250 WORDS)
    Citation
    Foetal and cancer patient fibroblasts produce an autocrine migration-stimulating factor not made by normal adult cells. 1988, 90 ( Pt 3):391-9 J Cell Sci
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/115970
    PubMed ID
    3253289
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Christie Publications

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    Related articles

    • Fibroblasts from cancer patients display a mixture of both foetal and adult-like phenotypic characteristics.
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