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    Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan.

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    Authors
    Sarrazin, S
    Lyon, Malcolm
    Deakin, Jon A
    Guerrini, M
    Lassalle, P
    Delehedde, M
    Lortat-Jacob, H
    Affiliation
    Institut de Biologie Structurale, UMR 5075 CNRS-CEA-UJF, 41 Rue Horowitz, 38027 Grenoble, France.
    Issue Date
    2010-11
    
    Metadata
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    Abstract
    Endocan is a recently identified soluble chondroitin/dermatan sulfate (CS/DS) proteoglycan. Synthesized by endothelial cells, it has been found to be over-expressed in the vasculature surrounding a number of tumors, and by promoting growth factor mitogenic activities, hepatocyte growth factor/scatter factor (HGF/SF) in particular, it supports cellular proliferation. In this work, we characterized the glycosaminoglycan (GAG) chain of Endocan, purified either from the naturally producing human umbilical vein endothelial cells (HUVEC) or from a recombinant over-expression system in human embryonic kidney cells (HEK). Compositional analysis using different chondroitinases as well as nuclear magnetic resonance studies revealed that the GAG chains from both sources share many characteristics, with the exception of size (15 and 40 kDa, respectively, for HUVEC and HEK-293 cells). The DS-specific, IdoA-containing disaccharides contribute 30% of the chain (15% of which are 2-O-sulfated) and are mostly clustered in tetra- (35%), hexa- (12%), and octa- (5%) saccharide domains. Highly sulfated D, E, and B disaccharide units (HexA2S-GalNAc6S, HexA-GalNAc4S6S, and HexA2S-GalNAc4S) were also detected in significant amounts in both chains and may account for the HGF/SF-binding activity of the CS/DS. This work establishes that HEK-293 cells can be engineered to provide a valuable source of Endocan with authentic CS/DS chains, enabling the purification of sufficient amounts for structural and/or binding analysis and providing a possible model of Endocan CS/DS chain organization.
    Citation
    Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan. 2010, 20 (11):1380-8 Glycobiology
    Journal
    Glycobiology
    URI
    http://hdl.handle.net/10541/115719
    DOI
    10.1093/glycob/cwq100
    PubMed ID
    20581009
    Type
    Article
    Language
    en
    ISSN
    1460-2423
    ae974a485f413a2113503eed53cd6c53
    10.1093/glycob/cwq100
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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