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    Expression of O6-Alkylguanine-DNA Alkyltransferase in Normal and Malignant Bladder Tissue of Egyptian Patients.

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    Authors
    Saad, A A
    Kassem, H S
    Povey, Andrew C
    Margison, Geoffrey P
    Affiliation
    Cancer Research UK Carcinogenesis Group, Paterson Institute for Cancer Research, The University of Manchester, Manchester M20 4BX, UK.
    Issue Date
    2010
    
    Metadata
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    Abstract
    Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O(6)-alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42-253 fmoles/mg protein and from 3.2-40 fmoles/μg DNA in normal and 58-468 fmoles/mg protein and 2.5-49.5 fmoles/mg protein, in the tumour tissues; only one tumour had undetectable activity. Pairwise comparison of MGMT activity in tumour and adjacent normal tissue showed no significant difference based on DNA content but was 1.75-fold higher in tumour (P < .01) based on protein. There was no effect of gender or bilharzia infection status. IF showed that in tumours, both the mean percentage of positive nuclei (57.3 ± 20.3%) and mean integrated IF (5.47 ± 3.66) were significantly higher than those in uninvolved tissues (42.8 ± 13.5% P = .04) and (1.89 ± 1.42; P < .01), respectively. These observations suggest that, overall, MGMT levels are increased during human bladder carcinogenesis and that MGMT downregulation is not a common feature of bladder cancers. Based on this, bladder cancers would be expected to be relatively resistant to chemotherapy which involved O(6)-guanine alkylating antitumour agents.
    Citation
    Expression of O6-Alkylguanine-DNA Alkyltransferase in Normal and Malignant Bladder Tissue of Egyptian Patients. 2010, 2010:840230 J Nucleic Acids
    Journal
    Journal of Nucleic Acids
    URI
    http://hdl.handle.net/10541/115711
    DOI
    10.4061/2010/840230
    PubMed ID
    20981358
    Type
    Article
    Language
    en
    ISSN
    2090-021X
    ae974a485f413a2113503eed53cd6c53
    10.4061/2010/840230
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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