Heterogeneity and cell cycle analyses from time-lapse studies of human keratinocytes in vitro.
AffiliationDepartment of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
MetadataShow full item record
AbstractWe have analysed the behaviour of cultured epidermal keratinocytes using time-lapse video recordings. We have found evidence for heterogeneity in the behaviour of the cells. Some lines underwent extensive self-renewal, thus expanding the population, while others produced daughter cells that migrated suprabasally and are presumed to have undergone terminal differentiation. We also present kinetic data on the cell cycle times, mitotic durations and post-mitotic residence times. The latter is the time between a cell's birth and eventual suprabasal migration. The data suggest that the 'decision' to migrate is in some way 'programmed' but that the actual migration is a stochastic process. Time-lapse analysis is a very powerful technique for lineage and cell kinetic analysis.
CitationHeterogeneity and cell cycle analyses from time-lapse studies of human keratinocytes in vitro. 1988, 89 ( Pt 3):359-64 J Cell Sci
JournalJournal of Cell Science
- Calcium-induced changes in cytoskeleton and motility of cultured human keratinocytes.
- Authors: Magee AI, Lytton NA, Watt FM
- Issue date: 1987 Sep
- Onset of epidermal differentiation in rapidly proliferating basal keratinocytes.
- Authors: Régnier M, Vaigot P, Darmon M, Pruniéras M
- Issue date: 1986 Oct
- Heterogeneity in the replicating population of cultured human epidermal keratinocytes.
- Authors: Albers KM, Setzer RW, Taichman LB
- Issue date: 1986
- Cell-cycle withdrawal in cultured keratinocytes.
- Authors: Albers KM, Greif F, Setzer RW, Taichman LB
- Issue date: 1987
- Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro.
- Authors: Leigh IM, Navsaria H, Purkis PE, McKay IA, Bowden PE, Riddle PN
- Issue date: 1995 Oct