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    The response of haemopoietic cells to growth factors: developmental implications of synergistic interactions.

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    Authors
    Heyworth, Clare M
    Ponting, Ian L
    Dexter, T Michael
    Affiliation
    Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
    Issue Date
    1988-10
    
    Metadata
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    Abstract
    Haemopoietic cell growth factors are normally assayed using unfractionated marrow cells (NBM). However, using this population it is difficult to distinguish between direct versus indirect effects, because of the low incidence of colony forming cells (CFC) and the presence of possible accessory cells (which may themselves be acted upon by the growth factors and stimulated to produce other growth stimulatory or inhibitory molecules that influence the development of the CFC). Furthermore, NBM contain the whole spectrum of multipotent and lineage-restricted CFC and it is often difficult to determine precisely which populations are being stimulated to develop. This latter problem can be solved, in part, by using marrow from mice previously treated with 5-fluorouracil (5-FU): an agent that preferentially kills the more mature, actively cycling CFC but spares the proliferatively quiescent multipotent stem cells. Since the 5-FU-treated marrow also contains many possible accessory cells, however, it is again not clear whether or not the responses elicited by growth factors are due to direct or indirect effects upon the CFC. To circumvent this problem we have obtained a highly enriched population of multipotent stem cells (FACS-BM) that is free of accessory cells, and have compared the responses of these cells to NBM and to 5-FU-BM in the presence of a variety of growth factors.(ABSTRACT TRUNCATED AT 250 WORDS)
    Citation
    The response of haemopoietic cells to growth factors: developmental implications of synergistic interactions. 1988, 91 ( Pt 2):239-47 J Cell Sci
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/115440
    PubMed ID
    3267697
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

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