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dc.contributor.authorHeyworth, Clare M
dc.contributor.authorDexter, T Michael
dc.date.accessioned2010-11-10T17:21:57Z
dc.date.available2010-11-10T17:21:57Z
dc.date.issued1988-01
dc.identifier.citationThe development of hemopoietic cells in response to stromal cells or growth factors is modified by agents that influence ADP-ribosylation. 1988, 2 (1):6-11 Leukemiaen
dc.identifier.issn0887-6924
dc.identifier.pmid3123809
dc.identifier.urihttp://hdl.handle.net/10541/115334
dc.description.abstractThe in vitro differentiation of multipotent stem cells in long-term marrow cultures can be blocked by treatment with agents that modify cholera toxin induced ADP-ribosylation of proteins. The latter agents also inhibit the growth and development of progenitor cells in soft gels in response to interleukin-3 but have little effect upon the development of progenitor cells that respond to the macrophage colony stimulating factor (CSF-1). Cholera toxin, in the same system, inhibits the development of CSF-1 responsive progenitor cells but has little effect on the development of cells that respond to IL-3. Similarly, progenitor cells that respond to IL-3 are relatively more resistant to pertussis toxin than cells that respond to CSF-1. These data indicate that ADP-ribosylation may be an important post-translational modification of regulatory proteins concerned with hemopoietic cell differentiation and growth in response to stromal cells or growth factors.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdenosine Diphosphate
dc.subject.meshAnimals
dc.subject.meshBone Marrow
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Differentiation
dc.subject.meshCells, Cultured
dc.subject.meshCholera Toxin
dc.subject.meshColony-Stimulating Factors
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshPertussis Toxin
dc.subject.meshRibose
dc.subject.meshVirulence Factors, Bordetella
dc.titleThe development of hemopoietic cells in response to stromal cells or growth factors is modified by agents that influence ADP-ribosylation.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester U.K.en
dc.identifier.journalLeukemiaen
html.description.abstractThe in vitro differentiation of multipotent stem cells in long-term marrow cultures can be blocked by treatment with agents that modify cholera toxin induced ADP-ribosylation of proteins. The latter agents also inhibit the growth and development of progenitor cells in soft gels in response to interleukin-3 but have little effect upon the development of progenitor cells that respond to the macrophage colony stimulating factor (CSF-1). Cholera toxin, in the same system, inhibits the development of CSF-1 responsive progenitor cells but has little effect on the development of cells that respond to IL-3. Similarly, progenitor cells that respond to IL-3 are relatively more resistant to pertussis toxin than cells that respond to CSF-1. These data indicate that ADP-ribosylation may be an important post-translational modification of regulatory proteins concerned with hemopoietic cell differentiation and growth in response to stromal cells or growth factors.


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