Increased O6-alkylguanine alkyltransferase activity in Chinese hamster V79 cells following selection with chloroethylating agents.
dc.contributor.author | Morten, John E N | |
dc.contributor.author | Margison, Geoffrey P | |
dc.date.accessioned | 2010-11-10T10:45:34Z | |
dc.date.available | 2010-11-10T10:45:34Z | |
dc.date.issued | 1988-01 | |
dc.identifier.citation | Increased O6-alkylguanine alkyltransferase activity in Chinese hamster V79 cells following selection with chloroethylating agents. 1988, 9 (1):45-9 Carcinogenesis | en |
dc.identifier.issn | 0143-3334 | |
dc.identifier.pmid | 3335046 | |
dc.identifier.doi | 10.1093/carcin/9.1.45 | |
dc.identifier.uri | http://hdl.handle.net/10541/115257 | |
dc.description.abstract | Chinese hamster V79 lung fibroblasts express low levels (specific activity 2-4 fmol/mg protein) of O6-alkylguanine (O6-AG) alkyltransferase (ATase). In cells surviving selection with low doses (10 micrograms/ml) of the chloroethylating agent, mitozolomide (Mz), ATase activity was increased 5- to 8-fold. Repeated selection of such cells produced a maximal specific activity of 36-40 fmol/mg protein, whilst selection at 20 or 40 micrograms/ml result in specific activities of approximately 50 and 70 fmol/mg respectively. Only slight decreases in ATase activity were seen by 51 days after an initial selection with 10 micrograms/ml Mz. A similar effect was observed using chlorozotocin. Selected cells had a higher D37 for Mz (2.5-6.0 micrograms/ml) in comparison with control cell (D37, 0.8 micrograms/ml) but the D37s for nitrogen mustard and vincristine were closely similar in selected and control cells. Possible explanations for the increase in ATase activity are discussed. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Cell Survival | |
dc.subject.mesh | Cricetinae | |
dc.subject.mesh | Cricetulus | |
dc.subject.mesh | Gene Expression Regulation | |
dc.subject.mesh | Lung | |
dc.subject.mesh | Methyltransferases | |
dc.subject.mesh | Nitrogen Mustard Compounds | |
dc.subject.mesh | O(6)-Methylguanine-DNA Methyltransferase | |
dc.subject.mesh | Transfection | |
dc.title | Increased O6-alkylguanine alkyltransferase activity in Chinese hamster V79 cells following selection with chloroethylating agents. | en |
dc.type | Article | en |
dc.contributor.department | Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK. | en |
dc.identifier.journal | Carcinogenesis | en |
html.description.abstract | Chinese hamster V79 lung fibroblasts express low levels (specific activity 2-4 fmol/mg protein) of O6-alkylguanine (O6-AG) alkyltransferase (ATase). In cells surviving selection with low doses (10 micrograms/ml) of the chloroethylating agent, mitozolomide (Mz), ATase activity was increased 5- to 8-fold. Repeated selection of such cells produced a maximal specific activity of 36-40 fmol/mg protein, whilst selection at 20 or 40 micrograms/ml result in specific activities of approximately 50 and 70 fmol/mg respectively. Only slight decreases in ATase activity were seen by 51 days after an initial selection with 10 micrograms/ml Mz. A similar effect was observed using chlorozotocin. Selected cells had a higher D37 for Mz (2.5-6.0 micrograms/ml) in comparison with control cell (D37, 0.8 micrograms/ml) but the D37s for nitrogen mustard and vincristine were closely similar in selected and control cells. Possible explanations for the increase in ATase activity are discussed. |