Show simple item record

dc.contributor.authorMorten, John E N
dc.contributor.authorMargison, Geoffrey P
dc.date.accessioned2010-11-10T10:45:34Z
dc.date.available2010-11-10T10:45:34Z
dc.date.issued1988-01
dc.identifier.citationIncreased O6-alkylguanine alkyltransferase activity in Chinese hamster V79 cells following selection with chloroethylating agents. 1988, 9 (1):45-9 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid3335046
dc.identifier.doi10.1093/carcin/9.1.45
dc.identifier.urihttp://hdl.handle.net/10541/115257
dc.description.abstractChinese hamster V79 lung fibroblasts express low levels (specific activity 2-4 fmol/mg protein) of O6-alkylguanine (O6-AG) alkyltransferase (ATase). In cells surviving selection with low doses (10 micrograms/ml) of the chloroethylating agent, mitozolomide (Mz), ATase activity was increased 5- to 8-fold. Repeated selection of such cells produced a maximal specific activity of 36-40 fmol/mg protein, whilst selection at 20 or 40 micrograms/ml result in specific activities of approximately 50 and 70 fmol/mg respectively. Only slight decreases in ATase activity were seen by 51 days after an initial selection with 10 micrograms/ml Mz. A similar effect was observed using chlorozotocin. Selected cells had a higher D37 for Mz (2.5-6.0 micrograms/ml) in comparison with control cell (D37, 0.8 micrograms/ml) but the D37s for nitrogen mustard and vincristine were closely similar in selected and control cells. Possible explanations for the increase in ATase activity are discussed.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshCell Survival
dc.subject.meshCricetinae
dc.subject.meshCricetulus
dc.subject.meshGene Expression Regulation
dc.subject.meshLung
dc.subject.meshMethyltransferases
dc.subject.meshNitrogen Mustard Compounds
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase
dc.subject.meshTransfection
dc.titleIncreased O6-alkylguanine alkyltransferase activity in Chinese hamster V79 cells following selection with chloroethylating agents.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
html.description.abstractChinese hamster V79 lung fibroblasts express low levels (specific activity 2-4 fmol/mg protein) of O6-alkylguanine (O6-AG) alkyltransferase (ATase). In cells surviving selection with low doses (10 micrograms/ml) of the chloroethylating agent, mitozolomide (Mz), ATase activity was increased 5- to 8-fold. Repeated selection of such cells produced a maximal specific activity of 36-40 fmol/mg protein, whilst selection at 20 or 40 micrograms/ml result in specific activities of approximately 50 and 70 fmol/mg respectively. Only slight decreases in ATase activity were seen by 51 days after an initial selection with 10 micrograms/ml Mz. A similar effect was observed using chlorozotocin. Selected cells had a higher D37 for Mz (2.5-6.0 micrograms/ml) in comparison with control cell (D37, 0.8 micrograms/ml) but the D37s for nitrogen mustard and vincristine were closely similar in selected and control cells. Possible explanations for the increase in ATase activity are discussed.


This item appears in the following Collection(s)

Show simple item record