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dc.contributor.authorHoey, Brigid M
dc.contributor.authorButler, John
dc.contributor.authorHalliwell, B
dc.date.accessioned2010-11-10T10:56:42Z
dc.date.available2010-11-10T10:56:42Z
dc.date.issued1988
dc.identifier.citationOn the specificity of allopurinol and oxypurinol as inhibitors of xanthine oxidase. A pulse radiolysis determination of rate constants for reaction of allopurinol and oxypurinol with hydroxyl radicals. 1988, 4 (4):259-63 Free Radic Res Communen
dc.identifier.issn8755-0199
dc.identifier.pmid2852627
dc.identifier.doi10.3109/10715768809055151
dc.identifier.urihttp://hdl.handle.net/10541/115235
dc.description.abstractAllopurinol has been employed as a "specific" inhibitor of xanthine oxidase in studies of hypoxic/reoxygenation injury. Pulse radiolysis was used to establish rate constants for the reactions of allopurinol and its major metabolite oxypurinol with hydroxyl radicals: values were (1.45 +/- 0.24) x 10(9) M-1 s-1 for allopurinol and (4.95 +/- 0.84) x 10(9) M-1 s-1 for oxypurinol. These rate constants show that, in view of the amounts of allopurinol that have been used in animal studies, hydroxyl radical scavenging by this molecule could contribute to its biological actions, especially if animals are pre-treated with allopurinol, so allowing oxypurinol to form. The ability of allopurinol to protect tissues not containing xanthine oxidase against reoxygenation injury may be related to radical scavenging by allopurinol and oxypurinol.
dc.language.isoenen
dc.subject.meshAllopurinol
dc.subject.meshFree Radicals
dc.subject.meshHydroxides
dc.subject.meshHydroxyl Radical
dc.subject.meshKinetics
dc.subject.meshOxypurinol
dc.subject.meshPyrimidines
dc.subject.meshXanthine Oxidase
dc.titleOn the specificity of allopurinol and oxypurinol as inhibitors of xanthine oxidase. A pulse radiolysis determination of rate constants for reaction of allopurinol and oxypurinol with hydroxyl radicals.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalFree Radical Research Communicationsen
html.description.abstractAllopurinol has been employed as a "specific" inhibitor of xanthine oxidase in studies of hypoxic/reoxygenation injury. Pulse radiolysis was used to establish rate constants for the reactions of allopurinol and its major metabolite oxypurinol with hydroxyl radicals: values were (1.45 +/- 0.24) x 10(9) M-1 s-1 for allopurinol and (4.95 +/- 0.84) x 10(9) M-1 s-1 for oxypurinol. These rate constants show that, in view of the amounts of allopurinol that have been used in animal studies, hydroxyl radical scavenging by this molecule could contribute to its biological actions, especially if animals are pre-treated with allopurinol, so allowing oxypurinol to form. The ability of allopurinol to protect tissues not containing xanthine oxidase against reoxygenation injury may be related to radical scavenging by allopurinol and oxypurinol.


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