On the specificity of allopurinol and oxypurinol as inhibitors of xanthine oxidase. A pulse radiolysis determination of rate constants for reaction of allopurinol and oxypurinol with hydroxyl radicals.
Affiliation
Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.Issue Date
1988
Metadata
Show full item recordAbstract
Allopurinol has been employed as a "specific" inhibitor of xanthine oxidase in studies of hypoxic/reoxygenation injury. Pulse radiolysis was used to establish rate constants for the reactions of allopurinol and its major metabolite oxypurinol with hydroxyl radicals: values were (1.45 +/- 0.24) x 10(9) M-1 s-1 for allopurinol and (4.95 +/- 0.84) x 10(9) M-1 s-1 for oxypurinol. These rate constants show that, in view of the amounts of allopurinol that have been used in animal studies, hydroxyl radical scavenging by this molecule could contribute to its biological actions, especially if animals are pre-treated with allopurinol, so allowing oxypurinol to form. The ability of allopurinol to protect tissues not containing xanthine oxidase against reoxygenation injury may be related to radical scavenging by allopurinol and oxypurinol.Citation
On the specificity of allopurinol and oxypurinol as inhibitors of xanthine oxidase. A pulse radiolysis determination of rate constants for reaction of allopurinol and oxypurinol with hydroxyl radicals. 1988, 4 (4):259-63 Free Radic Res CommunJournal
Free Radical Research CommunicationsDOI
10.3109/10715768809055151PubMed ID
2852627Type
ArticleLanguage
enISSN
8755-0199ae974a485f413a2113503eed53cd6c53
10.3109/10715768809055151
Scopus Count
Collections
Related articles
- Role of xanthine oxidase inhibitor as free radical scavenger: a novel mechanism of action of allopurinol and oxypurinol in myocardial salvage.
- Authors: Das DK, Engelman RM, Clement R, Otani H, Prasad MR, Rao PS
- Issue date: 1987 Oct 14
- Oxypurinol as an inhibitor of xanthine oxidase-catalyzed production of superoxide radical.
- Authors: Spector T
- Issue date: 1988 Jan 15
- Allopurinol and oxypurinol are hydroxyl radical scavengers.
- Authors: Moorhouse PC, Grootveld M, Halliwell B, Quinlan JG, Gutteridge JM
- Issue date: 1987 Mar 9
- Myocardial salvage by the free radical scavenging actions of xanthine oxidase inhibitors.
- Authors: Das DK, Engelman RM, Clement R, Otani H, Prasad MR, Rao PS
- Issue date: 1988
- Free radicals and cardioplegia: allopurinol and oxypurinol reduce myocardial injury following ischemic arrest.
- Authors: Chambers DJ, Braimbridge MV, Hearse DJ
- Issue date: 1987 Sep