The effect of a native collagen gel substratum on the synthesis of collagen by bovine brain capillary endothelial cells.
AffiliationStrangeways Research Laboratory, Worts Causeway, Cambridge, U.K.
MetadataShow full item record
AbstractCultured capillary endothelial cells, derived from bovine brain, and maintained on a plastic substratum synthesized predominantly interstitial collagens of which approximately 75 per cent were secreted into the medium. When grown on a native hydrated collagen type I gel, although no marked alteration in the 'collagen synthetic pattern' was observed, the overall level of collagen synthesis was increased by approximately 100 per cent. More dramatic, however, was the alteration in the distribution of these molecules between medium and cell layer. Interstitial collagens produced by cells grown on collagen gels were almost exclusively associated with the cell layer or collagenous gel. These studies, thus, demonstrate that an extracellular matrix may exert a considerable influence on the cellular synthetic activities and possibly cellular polarity of capillary endothelial cells.
CitationThe effect of a native collagen gel substratum on the synthesis of collagen by bovine brain capillary endothelial cells. 1988, 6 (3):209-15 Cell Biochem Funct
JournalCell Biochemistry and Function
- Synergistic effects of vascular endothelial growth factor and basic fibroblast growth factor on the proliferation and cord formation of bovine capillary endothelial cells within collagen gels.
- Authors: Goto F, Goto K, Weindel K, Folkman J
- Issue date: 1993 Nov
- Endothelial cell-mediated type I collagen gel contraction is regulated by hemin.
- Authors: Liu XD, Skold M, Umino T, Zhu YK, Romberger DJ, Spurzem JR, Rennard SI
- Issue date: 2000 Aug
- Reconstituted basement-membrane matrix modulates fibroblast activities in vitro.
- Authors: Emonard H, Grimaud JA, Nusgens B, Lapière CM, Foidart JM
- Issue date: 1987 Oct
- Substratum influence on collagen and fibronectin biosynthesis by arterial smooth muscle cells in vitro.
- Authors: Holderbaum D, Ehrhart LA
- Issue date: 1986 Feb
- Suramin inhibits C6 glioma-induced angiogenesis in vitro.
- Authors: Coomber BL
- Issue date: 1995 Jun