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dc.contributor.authorWren, Fiona E
dc.contributor.authorSchor, Ana M
dc.contributor.authorSchor, Seth L
dc.contributor.authorGrant, M E
dc.date.accessioned2010-11-09T15:26:09Z
dc.date.available2010-11-09T15:26:09Z
dc.date.issued1986-05
dc.identifier.citationModulation of smooth muscle cell behaviour by platelet-derived factors and the extracellular matrix. 1986, 127 (2):297-302 J Cell Physiolen
dc.identifier.issn0021-9541
dc.identifier.pmid3700485
dc.identifier.doi10.1002/jcp.1041270217
dc.identifier.urihttp://hdl.handle.net/10541/115153
dc.description.abstractWe have studied the combined effects of platelet-derived soluble factors and three types of macromolecular substrata on the proliferation and migration of smooth muscle cells in vitro. Bovine aortic smooth muscle cells were plated onto three-dimensional gels of type I collagen or onto cell-free extracellular matrices deposited on such gels by either bovine aortic endothelial cells or smooth muscle cells. The cells were cultured in the presence of whole-blood serum (WBS) or platelet-poor plasma (PPP). Smooth muscle cell proliferation on type I collagen gels was dependent on the presence of platelet-derived factors, i.e. the cells proliferated in the presence of WBS but not in PPP. In contrast, cell proliferation on the extracellular matrices occurred at the same rate in PPP and WBS. Smooth muscle cells plated onto collagen gels rapidly migrated down into the gel matrix; the percentage of cells migrating was inversely proportional to cell density. The presence of extracellular matrices did not alter the rate of cell migration into the underlying gel matrix. Irrespective of the substratum used, smooth muscle cell migration was independent of platelet-derived or plasma factors and occurred in the absence of proliferation. These results indicate that possible chemotactic, chemokinetic, and/or mitogenic factors produced by the vascular cells and deposited within the extracellular matrix may play an important role in modulating smooth muscle cell behaviour in the vascular wall.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCattle
dc.subject.meshCell Division
dc.subject.meshCell Movement
dc.subject.meshCollagen
dc.subject.meshExtracellular Matrix
dc.subject.meshGels
dc.subject.meshMuscle, Smooth, Vascular
dc.subject.meshPlatelet-Derived Growth Factor
dc.titleModulation of smooth muscle cell behaviour by platelet-derived factors and the extracellular matrix.en
dc.typeArticleen
dc.identifier.eissn1097-4652
dc.contributor.departmentCancer Research Campaign Laboratory of Medical Oncology, Christie Hospital Radium Institute, Manchester, M20 9BX, England.en
dc.identifier.journalJournal of Cellular Physiologyen
html.description.abstractWe have studied the combined effects of platelet-derived soluble factors and three types of macromolecular substrata on the proliferation and migration of smooth muscle cells in vitro. Bovine aortic smooth muscle cells were plated onto three-dimensional gels of type I collagen or onto cell-free extracellular matrices deposited on such gels by either bovine aortic endothelial cells or smooth muscle cells. The cells were cultured in the presence of whole-blood serum (WBS) or platelet-poor plasma (PPP). Smooth muscle cell proliferation on type I collagen gels was dependent on the presence of platelet-derived factors, i.e. the cells proliferated in the presence of WBS but not in PPP. In contrast, cell proliferation on the extracellular matrices occurred at the same rate in PPP and WBS. Smooth muscle cells plated onto collagen gels rapidly migrated down into the gel matrix; the percentage of cells migrating was inversely proportional to cell density. The presence of extracellular matrices did not alter the rate of cell migration into the underlying gel matrix. Irrespective of the substratum used, smooth muscle cell migration was independent of platelet-derived or plasma factors and occurred in the absence of proliferation. These results indicate that possible chemotactic, chemokinetic, and/or mitogenic factors produced by the vascular cells and deposited within the extracellular matrix may play an important role in modulating smooth muscle cell behaviour in the vascular wall.


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