The phase behaviour of dispersions of Bis-Azo PC: photoregulation of bilayer dynamics via lipid photochromism.
Affiliation
Department of Biological Sciences, University of Salford, U.K.Issue Date
1987-10-16
Metadata
Show full item recordAbstract
A phospholipid, 1,2-bis(4-(n-butyl)phenylazo-4'-phenylbutyroyl)phosphatidylcholine (Bis-Azo PC), has been synthesised and shown to form stable bilayer vesicles. Light-scattering measurements and differential scanning calorimetry show that a dispersion of the lipid has a cooperative phase transition at a similar temperature to that of dipalmitoylphosphatidylcholine, which Bis-Azo PC resembles in overall size. The phase behaviour of Bis-Azo PC has been investigated by fluorescence spectroscopy and using a series of spin-labelled fatty acid probes. Fluorescence measurements using chlorophyll a as probe sense the onset of the cooperative phase transition, but this is not clearly revealed by any of the spin probes tested. Hysteresis in the phase transition is detected both by light scattering measurements and by fluorescence spectroscopy. No transition is observed for a lipid analogue having a palmitic acid chain and a single azo-containing substituent. Bis-Azo PC is reversibly photochromic, isomerising on exposure to ultraviolet light to a photostationary state mixture where cis isomer predominates. Electron microscopy shows that photoisomerisation decreases average vesicle size, and light scattering and calorimetry demonstrate that the cooperative phase transition is abolished. Illumination with visible light establishes a new photostationary state where trans isomer predominates, and the phase transition is restored. The ability to modulate bilayer phase behaviour reversibly has possible application to relaxation studies of bilayer membrane function, and to drug delivery research.Citation
The phase behaviour of dispersions of Bis-Azo PC: photoregulation of bilayer dynamics via lipid photochromism. 1987, 903 (3):495-503 Biochim Biophys ActaJournal
Biochimica et Biophysica ActaDOI
10.1016/0005-2736(87)90056-3PubMed ID
2822108Type
ArticleLanguage
enISSN
0006-3002ae974a485f413a2113503eed53cd6c53
10.1016/0005-2736(87)90056-3
Scopus Count
Collections
Related articles
- Liposome fusion and lipid exchange on ultraviolet irradiation of liposomes containing a photochromic phospholipid.
- Authors: Morgan CG, Yianni YP, Sandhu SS, Mitchell AC
- Issue date: 1995 Jul
- A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: comparison with their 5-cholesten analogues.
- Authors: Benesch MG, Mannock DA, Lewis RN, McElhaney RN
- Issue date: 2014 Jan
- Light-induced fusion of liposomes with release of trapped marker dye is sensitised by photochromic phospholipid.
- Authors: Morgan CG, Thomas EW, Sandhu SS, Yianni YP, Mitchell AC
- Issue date: 1987 Oct 16
- Light-Controlled Lipid Interaction and Membrane Organization in Photolipid Bilayer Vesicles.
- Authors: Urban P, Pritzl SD, Konrad DB, Frank JA, Pernpeintner C, Roeske CR, Trauner D, Lohmüller T
- Issue date: 2018 Nov 6
- Interdigitation of phosphatidylcholine and phosphatidylethanolamine mixed with complexes of acidic lipids and polymyxin B or polymyxin B nonapeptide.
- Authors: Boggs JM, Wang HY, Rangaraj G, Tümmler B
- Issue date: 1989 Oct 16