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dc.contributor.authorCanfield, Ann E
dc.contributor.authorSchor, Ana M
dc.contributor.authorSchor, Seth L
dc.contributor.authorGrant, Michael E
dc.date.accessioned2010-11-08T16:18:00Z
dc.date.available2010-11-08T16:18:00Z
dc.date.issued1986-04-15
dc.identifier.citationThe biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes. 1986, 235 (2):375-83 Biochem Jen
dc.identifier.issn0264-6021
dc.identifier.pmid3741397
dc.identifier.urihttp://hdl.handle.net/10541/115015
dc.description.abstractPrevious studies have indicated that the morphology and behaviour of bovine retinal microvessel endothelial cells are influenced by culture conditions in vitro. Data are presented here concerning the biosynthesis of matrix macromolecules by bovine retinal endothelial cells cultured under conditions in which the cells display either the 'cobblestone' or the 'sprouting' phenotype. Newly synthesized matrix proteins were identified by their characteristic electrophoretic mobilities, immunoprecipitation with specific antibodies, susceptibilities to enzymic digestions and chromatographic behaviour. Type IV procollagen was the major collagenous species synthesized by early-passage cells forming a 'cobblestone' monolayer. In contrast, cells displaying the 'sprouting' morphology switched to the predominant synthesis of interstitial fibrillar collagens (types I and III). Fibronectin was synthesized by retinal endothelial cells under all the experimental conditions studied. A non-collagenous glycoprotein of Mr approx. 47,000 was also a major biosynthetic product of these cells. The synthesis of thrombospondin was very much dependent on the nature of the substratum on which the cells were cultured. This glycoprotein was synthesized in large amounts by 'cobblestone' endothelial cells cultured on gelatin-coated dishes, whereas its synthesis was markedly decreased by culturing the cells on collagen gels, and the protein appeared to be absent when the cells were plated within collagen gels ('sprouting' cells). Late-passage retinal cells synthesized predominantly type I procollagen, variable amounts of type III procollagen and only traces of type IV procollagen, irrespective of whether the cells displayed a 'cobblestone' or 'sprouting' morphology.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshCattle
dc.subject.meshCells, Cultured
dc.subject.meshCollagen
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshEndothelium
dc.subject.meshExtracellular Matrix
dc.subject.meshEye Proteins
dc.subject.meshGene Expression Regulation
dc.subject.meshMacromolecular Substances
dc.subject.meshPhenotype
dc.subject.meshRetina
dc.subject.meshTunicamycin
dc.titleThe biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes.en
dc.typeArticleen
dc.identifier.eissn1470-8728
dc.contributor.departmentDepartment of Biochemistry, University of Manchester Medical School, Manchester M13 9PT, U.K.en
dc.identifier.journalThe Biochemical Journalen
html.description.abstractPrevious studies have indicated that the morphology and behaviour of bovine retinal microvessel endothelial cells are influenced by culture conditions in vitro. Data are presented here concerning the biosynthesis of matrix macromolecules by bovine retinal endothelial cells cultured under conditions in which the cells display either the 'cobblestone' or the 'sprouting' phenotype. Newly synthesized matrix proteins were identified by their characteristic electrophoretic mobilities, immunoprecipitation with specific antibodies, susceptibilities to enzymic digestions and chromatographic behaviour. Type IV procollagen was the major collagenous species synthesized by early-passage cells forming a 'cobblestone' monolayer. In contrast, cells displaying the 'sprouting' morphology switched to the predominant synthesis of interstitial fibrillar collagens (types I and III). Fibronectin was synthesized by retinal endothelial cells under all the experimental conditions studied. A non-collagenous glycoprotein of Mr approx. 47,000 was also a major biosynthetic product of these cells. The synthesis of thrombospondin was very much dependent on the nature of the substratum on which the cells were cultured. This glycoprotein was synthesized in large amounts by 'cobblestone' endothelial cells cultured on gelatin-coated dishes, whereas its synthesis was markedly decreased by culturing the cells on collagen gels, and the protein appeared to be absent when the cells were plated within collagen gels ('sprouting' cells). Late-passage retinal cells synthesized predominantly type I procollagen, variable amounts of type III procollagen and only traces of type IV procollagen, irrespective of whether the cells displayed a 'cobblestone' or 'sprouting' morphology.


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