• Login
    View Item 
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    •   Home
    • The Christie Research Publications Repository
    • All Christie Publications
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    The biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Canfield, Ann E
    Schor, Ana M
    Schor, Seth L
    Grant, Michael E
    Affiliation
    Department of Biochemistry, University of Manchester Medical School, Manchester M13 9PT, U.K.
    Issue Date
    1986-04-15
    
    Metadata
    Show full item record
    Abstract
    Previous studies have indicated that the morphology and behaviour of bovine retinal microvessel endothelial cells are influenced by culture conditions in vitro. Data are presented here concerning the biosynthesis of matrix macromolecules by bovine retinal endothelial cells cultured under conditions in which the cells display either the 'cobblestone' or the 'sprouting' phenotype. Newly synthesized matrix proteins were identified by their characteristic electrophoretic mobilities, immunoprecipitation with specific antibodies, susceptibilities to enzymic digestions and chromatographic behaviour. Type IV procollagen was the major collagenous species synthesized by early-passage cells forming a 'cobblestone' monolayer. In contrast, cells displaying the 'sprouting' morphology switched to the predominant synthesis of interstitial fibrillar collagens (types I and III). Fibronectin was synthesized by retinal endothelial cells under all the experimental conditions studied. A non-collagenous glycoprotein of Mr approx. 47,000 was also a major biosynthetic product of these cells. The synthesis of thrombospondin was very much dependent on the nature of the substratum on which the cells were cultured. This glycoprotein was synthesized in large amounts by 'cobblestone' endothelial cells cultured on gelatin-coated dishes, whereas its synthesis was markedly decreased by culturing the cells on collagen gels, and the protein appeared to be absent when the cells were plated within collagen gels ('sprouting' cells). Late-passage retinal cells synthesized predominantly type I procollagen, variable amounts of type III procollagen and only traces of type IV procollagen, irrespective of whether the cells displayed a 'cobblestone' or 'sprouting' morphology.
    Citation
    The biosynthesis of extracellular-matrix components by bovine retinal endothelial cells displaying distinctive morphological phenotypes. 1986, 235 (2):375-83 Biochem J
    Journal
    The Biochemical Journal
    URI
    http://hdl.handle.net/10541/115015
    PubMed ID
    3741397
    Type
    Article
    Language
    en
    ISSN
    0264-6021
    EISSN
    1470-8728
    Collections
    All Christie Publications

    entitlement

    Related articles

    • Heterogeneity in collagen biosynthesis by sprouting retinal endothelial cells.
    • Authors: Canfield AE, Schor AM
    • Issue date: 1994 Apr
    • Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum.
    • Authors: Canfield AE, Boot-Handford RP, Schor AM
    • Issue date: 1990 May 15
    • The response of endothelial cells to TGF beta-1 is dependent upon cell shape, proliferative state and the nature of the substratum.
    • Authors: Sutton AB, Canfield AE, Schor SL, Grant ME, Schor AM
    • Issue date: 1991 Aug
    • Modulation of extracellular matrix biosynthesis by bovine retinal pericytes in vitro: effects of the substratum and cell density.
    • Authors: Canfield AE, Allen TD, Grant ME, Schor SL, Schor AM
    • Issue date: 1990 May
    • Identification and partial characterization of two major proteins of Mr 47,000 synthesized by bovine retinal endothelial cells in culture.
    • Authors: Canfield AE, Schor AM, West DC, Schor SL, Grant ME
    • Issue date: 1987 Aug 15
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.