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dc.contributor.authorChristmas, Stephen E
dc.contributor.authorMeager, A
dc.contributor.authorMoore, Michael
dc.date.accessioned2010-11-08T11:41:17Z
dc.date.available2010-11-08T11:41:17Z
dc.date.issued1987-08
dc.identifier.citationProduction of interferon and tumour necrosis factor by cloned human natural cytotoxic lymphocytes and T cells. 1987, 69 (2):441-50 Clin. Exp. Immunol.en
dc.identifier.issn0009-9104
dc.identifier.pmid2443292
dc.identifier.urihttp://hdl.handle.net/10541/114945
dc.description.abstractCell lines and clones, derived from natural killer (NK) cell-enriched (B73.1+) peripheral blood lymphocytes (PBL) from several human donors, that expressed distinct surface phenotypes and were cytolytically active against K562 target cells were tested for their capacity to produce interferon (IFN) and tumour necrosis factor (TNF), IFN and TNF were measured firstly in biological assays and secondly in specific immunoassays for alpha-IFN, gamma-IFN and tumour necrosis factor (TNF alpha). It was found that the majority of NK-derived lines and clones were highly cytotoxic towards K562, but generally produced relatively low or undetectable levels of gamma-IFN and TNF alpha following stimulation with phytohaemagglutinin. No alpha-IFN was detected in supernatants from these cells. In comparison, cell lines and clones, derived from T lymphocyte (B73.1-) enriched PBL from the same donors were poorly cytotoxic towards K562, but generally produced higher levels of gamma-IFN and TNF than NK-derived cells. Thus, neither gamma-IFN nor TNF production were shown to correlate well with the capacity of NK-derived or T cell clones to effect cytotoxic action towards K562 in vitro. These results suggest that the co-production of gamma-IFN and TNF is not indicative of cytotoxic potential.
dc.language.isoenen
dc.subjectTumour Necrosis Factor-alphaen
dc.subject.meshAntigens, Surface
dc.subject.meshBiological Assay
dc.subject.meshClone Cells
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshHumans
dc.subject.meshInterferon-gamma
dc.subject.meshInterferons
dc.subject.meshKiller Cells, Natural
dc.subject.meshT-Lymphocytes
dc.subject.meshTumor Necrosis Factor-alpha
dc.titleProduction of interferon and tumour necrosis factor by cloned human natural cytotoxic lymphocytes and T cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalClinical and Experimental Immunologyen
html.description.abstractCell lines and clones, derived from natural killer (NK) cell-enriched (B73.1+) peripheral blood lymphocytes (PBL) from several human donors, that expressed distinct surface phenotypes and were cytolytically active against K562 target cells were tested for their capacity to produce interferon (IFN) and tumour necrosis factor (TNF), IFN and TNF were measured firstly in biological assays and secondly in specific immunoassays for alpha-IFN, gamma-IFN and tumour necrosis factor (TNF alpha). It was found that the majority of NK-derived lines and clones were highly cytotoxic towards K562, but generally produced relatively low or undetectable levels of gamma-IFN and TNF alpha following stimulation with phytohaemagglutinin. No alpha-IFN was detected in supernatants from these cells. In comparison, cell lines and clones, derived from T lymphocyte (B73.1-) enriched PBL from the same donors were poorly cytotoxic towards K562, but generally produced higher levels of gamma-IFN and TNF than NK-derived cells. Thus, neither gamma-IFN nor TNF production were shown to correlate well with the capacity of NK-derived or T cell clones to effect cytotoxic action towards K562 in vitro. These results suggest that the co-production of gamma-IFN and TNF is not indicative of cytotoxic potential.


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