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    Production of interferon and tumour necrosis factor by cloned human natural cytotoxic lymphocytes and T cells.

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    Authors
    Christmas, Stephen E
    Meager, A
    Moore, Michael
    Affiliation
    Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
    Issue Date
    1987-08
    
    Metadata
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    Abstract
    Cell lines and clones, derived from natural killer (NK) cell-enriched (B73.1+) peripheral blood lymphocytes (PBL) from several human donors, that expressed distinct surface phenotypes and were cytolytically active against K562 target cells were tested for their capacity to produce interferon (IFN) and tumour necrosis factor (TNF), IFN and TNF were measured firstly in biological assays and secondly in specific immunoassays for alpha-IFN, gamma-IFN and tumour necrosis factor (TNF alpha). It was found that the majority of NK-derived lines and clones were highly cytotoxic towards K562, but generally produced relatively low or undetectable levels of gamma-IFN and TNF alpha following stimulation with phytohaemagglutinin. No alpha-IFN was detected in supernatants from these cells. In comparison, cell lines and clones, derived from T lymphocyte (B73.1-) enriched PBL from the same donors were poorly cytotoxic towards K562, but generally produced higher levels of gamma-IFN and TNF than NK-derived cells. Thus, neither gamma-IFN nor TNF production were shown to correlate well with the capacity of NK-derived or T cell clones to effect cytotoxic action towards K562 in vitro. These results suggest that the co-production of gamma-IFN and TNF is not indicative of cytotoxic potential.
    Citation
    Production of interferon and tumour necrosis factor by cloned human natural cytotoxic lymphocytes and T cells. 1987, 69 (2):441-50 Clin. Exp. Immunol.
    Journal
    Clinical and Experimental Immunology
    URI
    http://hdl.handle.net/10541/114945
    PubMed ID
    2443292
    Type
    Article
    Language
    en
    ISSN
    0009-9104
    Collections
    All Paterson Institute for Cancer Research

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