Differentiated HL-60 promyelocytic leukaemia cells produce a factor inducing differentiation.
| dc.contributor.author | Djulbegović, Benjamin | |
| dc.contributor.author | Christmas, Stephen E | |
| dc.contributor.author | Moore, Michael | |
| dc.date.accessioned | 2010-11-08T10:08:31Z | |
| dc.date.available | 2010-11-08T10:08:31Z | |
| dc.date.issued | 1987 | |
| dc.identifier.citation | Differentiated HL-60 promyelocytic leukaemia cells produce a factor inducing differentiation. 1987, 11 (3):259-64 Leuk. Res. | en |
| dc.identifier.issn | 0145-2126 | |
| dc.identifier.pmid | 3470575 | |
| dc.identifier.doi | 10.1016/0145-2126(87)90049-X | |
| dc.identifier.uri | http://hdl.handle.net/10541/114916 | |
| dc.description.abstract | The bipotential human promyelocytic leukaemia cell line HL-60 can be induced to differentiate into monocytic or granulocytic cells by treatment with 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) or dimethylsulphoxide (DMSO) respectively. Conditioned media (CM) from 1,25(OH)2D3- or DMSO-treated cells were able to induce monocytic differentiation in fresh HL-60 cells as measured by induction of non-specific esterase and macrophage surface markers. CM from 1,25(OH)2D3-treated cells also led to a dose dependent loss of proliferative capacity in soft agar colony assays. These effects were not due to a toxic effect of the CM or to residual inducer present in the CM. gamma-interferon and GM-CSF were apparently not responsible for these effects. CM from the human histiocytic lymphoma cell line U937 led to only a low level of induction of macrophage differentiation in fresh HL-60 cells. The defect in HL-60 leukaemic cells may therefore be at the level of induction of an autonomously-produced differentiation factor. | |
| dc.language.iso | en | en |
| dc.subject | Acute Myeloid Leukaemia | en |
| dc.subject.mesh | Calcitriol | |
| dc.subject.mesh | Cell Differentiation | |
| dc.subject.mesh | Cell Division | |
| dc.subject.mesh | Cell Line | |
| dc.subject.mesh | Culture Media | |
| dc.subject.mesh | Dimethyl Sulfoxide | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Leukemia, Myeloid, Acute | |
| dc.subject.mesh | Macrophages | |
| dc.title | Differentiated HL-60 promyelocytic leukaemia cells produce a factor inducing differentiation. | en |
| dc.type | Article | en |
| dc.identifier.journal | Leukemia Research | en |
| html.description.abstract | The bipotential human promyelocytic leukaemia cell line HL-60 can be induced to differentiate into monocytic or granulocytic cells by treatment with 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) or dimethylsulphoxide (DMSO) respectively. Conditioned media (CM) from 1,25(OH)2D3- or DMSO-treated cells were able to induce monocytic differentiation in fresh HL-60 cells as measured by induction of non-specific esterase and macrophage surface markers. CM from 1,25(OH)2D3-treated cells also led to a dose dependent loss of proliferative capacity in soft agar colony assays. These effects were not due to a toxic effect of the CM or to residual inducer present in the CM. gamma-interferon and GM-CSF were apparently not responsible for these effects. CM from the human histiocytic lymphoma cell line U937 led to only a low level of induction of macrophage differentiation in fresh HL-60 cells. The defect in HL-60 leukaemic cells may therefore be at the level of induction of an autonomously-produced differentiation factor. |