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dc.contributor.authorMatera, L
dc.contributor.authorCardoso, E
dc.contributor.authorCaudana, L
dc.contributor.authorMoore, Michael
dc.date.accessioned2010-11-06T12:49:01Z
dc.date.available2010-11-06T12:49:01Z
dc.date.issued1987-02
dc.identifier.citationGeneration of non MHC restricted killing in non-cytotoxic T-lymphocytes from leukaemia patients. 1987, 65 (2):151-7 Br J Haematolen
dc.identifier.issn0007-1048
dc.identifier.pmid2950919
dc.identifier.doi10.1111/j.1365-2141.1987.tb02257.x
dc.identifier.urihttp://hdl.handle.net/10541/114850
dc.description.abstractExperiments were undertaken to determine whether the depression of natural killer (NK) activity previously observed in the peripheral blood lymphocytes (PBL) of leukaemia patients in remission extended to NK-like precursors in the blood. T-lymphocytes (E+) from leukaemia patients and normal subjects were depleted of IgG Fc-receptor-bearing (gamma FcR) fresh NK cells by passage over immune complex-coated monolayers. gamma FcR - E + PBL were cultured alone or with DAUDI cells. On day 5 of culture, cytotoxicity toward the NK-sensitive cell lines K562 and MOLT-4 was evaluated in the responder lymphocytes of leukaemia patients and controls. Negligible NK-like cytotoxicity was found in both FcR - E + PBL responder populations cultured alone. By contrast, stimulation with DAUDI induced high levels of K562 and MOLT-4 cytotoxicity in leukaemia as well as in normal responder cells. Complement-mediated cytotoxicity experiments using various McAb demonstrated that in both normal and leukaemia cultures NK-like effectors react with the pan-T OKT3 McAb and with the OKT11 McAB directed to the SRBC receptor, but not with Leu 1 1b and OKM1 McAbs, directed against antigens expressed on peripheral blood NK cells. Fractionation of the responder cells on discontinuous Percoll gradients showed that most of this activity was present in the highly dividing blast cell fraction.
dc.language.isoenen
dc.subjectLeukaemiaen
dc.subjectAcute Erythroblastic Leukaemiaen
dc.subject.meshAcute Disease
dc.subject.meshCytotoxicity, Immunologic
dc.subject.meshHLA Antigens
dc.subject.meshHumans
dc.subject.meshKiller Cells, Natural
dc.subject.meshLeukemia
dc.subject.meshLeukemia, Erythroblastic, Acute
dc.subject.meshLymphocyte Culture Test, Mixed
dc.subject.meshReceptors, Fc
dc.subject.meshReceptors, IgG
dc.subject.meshT-Lymphocytes
dc.titleGeneration of non MHC restricted killing in non-cytotoxic T-lymphocytes from leukaemia patients.en
dc.typeArticleen
dc.identifier.journalBritish Journal of Haematologyen
html.description.abstractExperiments were undertaken to determine whether the depression of natural killer (NK) activity previously observed in the peripheral blood lymphocytes (PBL) of leukaemia patients in remission extended to NK-like precursors in the blood. T-lymphocytes (E+) from leukaemia patients and normal subjects were depleted of IgG Fc-receptor-bearing (gamma FcR) fresh NK cells by passage over immune complex-coated monolayers. gamma FcR - E + PBL were cultured alone or with DAUDI cells. On day 5 of culture, cytotoxicity toward the NK-sensitive cell lines K562 and MOLT-4 was evaluated in the responder lymphocytes of leukaemia patients and controls. Negligible NK-like cytotoxicity was found in both FcR - E + PBL responder populations cultured alone. By contrast, stimulation with DAUDI induced high levels of K562 and MOLT-4 cytotoxicity in leukaemia as well as in normal responder cells. Complement-mediated cytotoxicity experiments using various McAb demonstrated that in both normal and leukaemia cultures NK-like effectors react with the pan-T OKT3 McAb and with the OKT11 McAB directed to the SRBC receptor, but not with Leu 1 1b and OKM1 McAbs, directed against antigens expressed on peripheral blood NK cells. Fractionation of the responder cells on discontinuous Percoll gradients showed that most of this activity was present in the highly dividing blast cell fraction.


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