A cell line from Wilms' tumour with deletion in short arm of chromosome II.
Affiliation
Christie Hospital, Manchester, UK.Issue Date
1987-10-15
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A cell line (T3/73) from a Wilms' tumour has been established from a 9 month-old boy with aniridia. The tumour was removed in 1973. On histological examination a diagnosis of Wilms' tumour was made which showed undifferentiated areas, marked tubule formation and abundant striped muscle fibres. The tumour cells, which are fusiform, grew rapidly in culture without the addition of growth factors, and have undergone over 100 passages. Approximately 95% and 5% were positive for desmin and cytokeratin, respectively. The cell doubling time was 28 hr. Cytogenetic studies revealed a karyotype of 46,XY,del(11) (p12::p14). Although the cells stained very intensely with a monoclonal antibody that detects oncogene ras p 21 antigen, Southern blot analysis using c-Ha-ras as a probe failed to reveal an obvious deletion or amplification of either Ha-ras allele.Citation
A cell line from Wilms' tumour with deletion in short arm of chromosome II. 1987, 40 (4):499-504 Int. J. CancerJournal
International Journal of Cancer.DOI
10.1002/ijc.2910400412PubMed ID
2444543Type
ArticleLanguage
enISSN
0020-7136ae974a485f413a2113503eed53cd6c53
10.1002/ijc.2910400412
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