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    Differential MHC class II expression on human peripheral blood monocytes and dendritic cells.

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    Authors
    Brooks, C F
    Moore, M
    Affiliation
    Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.
    Issue Date
    1988-02
    
    Metadata
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    Abstract
    Both monocytes (MO) and dendritic cells (DC) in human peripheral blood are of a plastic-adherent nature. The expression of the MHC class II sublocus products HLA-DP, -DQ and -DR on human peripheral blood transiently adherent cells (TA) was examined by an immunocytochemical staining technique. While most TA showed strong expression of molecules of the HLA-DR subtype, only a small proportion of cells (2-6%) showed strong HLA-DP or -DQ positivity. This strong expression of the HLA-DP and HLA-DQ sublocus products by a subset of TA was seen only after short-term culture; freshly isolated cells expressed comparatively low levels of these molecules. Enrichment for Fc receptor-negative or low-density cells from TA produced populations with strong HLA-DQ and -DP expression. Such co-enrichment of the strongly HLA-DQ+ and strongly HLA-DP+ cells suggests that the same cells express high levels of both types of MHC class II molecule. Immunocytochemical analysis of TA indicated that the strongly HLA-DQ+ cells, at least, were only weakly or non-reactive with the MO-specific monoclonal antibodies OKM1, UCHM1, MO2 and EB11. In addition, strongly HLA-DQ- or -DP-positive cells were poorly phagocytic in comparison with the majority of adherent cells. The apparent FcR-negative, low-density and weakly phagocytic nature of the strongly HLA-DQ/DP+ cells, combined with their lack of reactivity with several MO-specific antibodies, suggests that they may represent the DC component of TA. Such strong HLA-DQ/DP expression by DC may aid their positive identification in human peripheral blood and may be of relevance to DC function in antigen presentation.
    Citation
    Differential MHC class II expression on human peripheral blood monocytes and dendritic cells. 1988, 63 (2):303-11 Immunology
    Journal
    Immunology
    URI
    http://hdl.handle.net/10541/114627
    PubMed ID
    3350576
    Type
    Article
    Language
    en
    ISSN
    0019-2805
    Collections
    All Paterson Institute for Cancer Research

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