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dc.contributor.authorWilliams, D A
dc.contributor.authorLim, B
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorLongtine, J
dc.contributor.authorDexter, T Michael
dc.date.accessioned2010-11-03T15:34:18Z
dc.date.available2010-11-03T15:34:18Z
dc.date.issued1988-06
dc.identifier.citationRestriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells. 1988, 71 (6):1738-43 Blooden
dc.identifier.issn0006-4971
dc.identifier.pmid2836004
dc.identifier.urihttp://hdl.handle.net/10541/114519
dc.description.abstractA recombinant retrovirus (DHFR*-SVADA) in which human adenosine deaminase (ADA) cDNA is transcribed from an internal SV40 promoter was used to infect murine hematopoietic stem and progenitor cells. Human ADA enzyme was not expressed in infected primary murine pluripotent stem cell-derived spleen or progenitor colonies (CFU-GM, CFU-Mix, BFU-E). In contrast, human ADA enzyme activity was readily detected in progenitor colonies derived from immortalized multipotent factor-dependent cells. The level of human enzyme was near endogenous murine enzyme levels and was equivalent in undifferentiated stem cells and differentiated myeloid, erythroid, and mixed colonies. These results indicate that cellular properties other than the stage of differentiation are important in determining the expression of foreign sequences introduced by retroviruses. Cell lines that are immortalized but still capable of induced differentiation may contain factors that abrogate blocks to expression that are manifested in primary hematopoietic stem cells.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdenosine Deaminase
dc.subject.meshCell Differentiation
dc.subject.meshDNA, Recombinant
dc.subject.meshGene Expression Regulation
dc.subject.meshGrowth Substances
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshRNA, Messenger
dc.subject.meshRetroviridae
dc.subject.meshSimian virus 40
dc.subject.meshTransduction, Genetic
dc.titleRestriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells.en
dc.typeArticleen
dc.contributor.departmentDivision of Hematology-Oncology, Howard Hughes Medical Institute, Boston, MA.en
dc.identifier.journalBlooden
html.description.abstractA recombinant retrovirus (DHFR*-SVADA) in which human adenosine deaminase (ADA) cDNA is transcribed from an internal SV40 promoter was used to infect murine hematopoietic stem and progenitor cells. Human ADA enzyme was not expressed in infected primary murine pluripotent stem cell-derived spleen or progenitor colonies (CFU-GM, CFU-Mix, BFU-E). In contrast, human ADA enzyme activity was readily detected in progenitor colonies derived from immortalized multipotent factor-dependent cells. The level of human enzyme was near endogenous murine enzyme levels and was equivalent in undifferentiated stem cells and differentiated myeloid, erythroid, and mixed colonies. These results indicate that cellular properties other than the stage of differentiation are important in determining the expression of foreign sequences introduced by retroviruses. Cell lines that are immortalized but still capable of induced differentiation may contain factors that abrogate blocks to expression that are manifested in primary hematopoietic stem cells.


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