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    Restriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells.

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    Authors
    Williams, D A
    Lim, B
    Spooncer, Elaine
    Longtine, J
    Dexter, T Michael
    Affiliation
    Division of Hematology-Oncology, Howard Hughes Medical Institute, Boston, MA.
    Issue Date
    1988-06
    
    Metadata
    Show full item record
    Abstract
    A recombinant retrovirus (DHFR*-SVADA) in which human adenosine deaminase (ADA) cDNA is transcribed from an internal SV40 promoter was used to infect murine hematopoietic stem and progenitor cells. Human ADA enzyme was not expressed in infected primary murine pluripotent stem cell-derived spleen or progenitor colonies (CFU-GM, CFU-Mix, BFU-E). In contrast, human ADA enzyme activity was readily detected in progenitor colonies derived from immortalized multipotent factor-dependent cells. The level of human enzyme was near endogenous murine enzyme levels and was equivalent in undifferentiated stem cells and differentiated myeloid, erythroid, and mixed colonies. These results indicate that cellular properties other than the stage of differentiation are important in determining the expression of foreign sequences introduced by retroviruses. Cell lines that are immortalized but still capable of induced differentiation may contain factors that abrogate blocks to expression that are manifested in primary hematopoietic stem cells.
    Citation
    Restriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells. 1988, 71 (6):1738-43 Blood
    Journal
    Blood
    URI
    http://hdl.handle.net/10541/114519
    PubMed ID
    2836004
    Type
    Article
    Language
    en
    ISSN
    0006-4971
    Collections
    All Paterson Institute for Cancer Research

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