Affiliation
Department of Medical Genetics, St Mary's Hospital, Manchester, UK.Issue Date
1988-08
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Two hybrid cell lines expressing human CD4 were prepared by fusing human B-lymphoid cells with the mouse T-lymphoma BW5147. Hybrid TF42 was derived from a human B-lymphoblastoid line and TF53.1 from a human B-ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence-activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4+ hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T-cell antigens screened on the CD4+ and CD4- variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4+ cells. These hybrids should be useful in the preparation, screening and analysis of anti-CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.Citation
Expression of human CD4 by two human-mouse interlineage hybrids. 1988, 15 (4):197-208 J. Immunogenet.Journal
Journal of ImmunogeneticsDOI
10.1111/j.1744-313X.1988.tb00422.xPubMed ID
2471746Type
ArticleLanguage
enISSN
0305-1811ae974a485f413a2113503eed53cd6c53
10.1111/j.1744-313X.1988.tb00422.x
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