Distribution of 5-doxylstearic acid in the membranes of mammalian cells.
Affiliation
University of Illinois College of Medicine, Urbana.Issue Date
1988-10-06
Metadata
Show full item recordAbstract
Concentration-dependent spin broadening of ESR spectra of the nitroxide 5-doxylstearic acid has been used to evaluate the distribution of 5-doxylstearic acid in the membranes of intact mouse thymus-bone marrow (TB) and Chinese hamster ovary (CHO) cells. TB cells, CHO cells, erythrocytes, and isolated plasma membranes from CHO cells were labelled with 5-doxylstearic acid and the peak to peak linewidths of the central line of the resulting ESR spectra were measured. The measured line widths were linearly dependent on the amount of 5-doxylstearic acid incorporated into the sample over the range of 0-0.18 mol nitroxide per mol lipid. In erythrocytes, the relationship between linewidths approximated a linear function at lower concentrations of 5-doxylstearic acid, up to 0.07 mol nitroxide per mol lipid. The amount of broadening of the central line for a given amount of 5-doxylstearic acid was far less for intact cells than for either erythrocytes or plasma membrane, indicating that the 5-doxylstearic acid samples a much larger lipid pool in the intact cells. With the broad assumption that the mobility of the 5-doxylstearic acid is similar in different membranes, the size of the lipid pool sampled by 5-doxylstearic acid is approximately equal to the total cellular lipid in intact cells. If a given concentration of 5-doxylstearic acid sampled only the plasma membrane of TB or CHO cells, we would expect to see a linewidth corresponding to a 12-20-fold greater local concentration of 5-doxylstearic acid than was observed, since the plasma membranes of CHO and TB cells represent only 5-8 percent of the total cellular lipid. Therefore, the 5-doxylstearic acid must distribute into most or all cellular membranes of intact cells and is not localized in the plasma membrane alone.Citation
Distribution of 5-doxylstearic acid in the membranes of mammalian cells. 1988, 944 (2):315-20 Biochim. Biophys. ActaJournal
Biochimica et Biophysica ActaPubMed ID
2846059Type
ArticleLanguage
enISSN
0006-3002Collections
Related articles
- Kinetics of distribution of 5-doxylstearic acid in mouse lymphocytes.
- Authors: Morse PD 2nd, Yuann JM
- Issue date: 1993 Jan-Feb
- Is the mammalian cell plasma membrane a barrier to oxygen transport?
- Authors: Subczynski WK, Hopwood LE, Hyde JS
- Issue date: 1992 Jul
- Azethoxyl nitroxide spin labels. ESR studies involving thiourea crystals, model membrane systems and chromatophores, and chemical reduction with ascorbate and dithiothreitol.
- Authors: Lee TD, Birrell GB, Bjorkman PJ, Keana JF
- Issue date: 1979 Feb 2
- Differences in the reduction kinetics of incorporated spin labels in undifferentiated and differentiated mouse neuroblastoma cells.
- Authors: Chen KY, McLaughlin MG
- Issue date: 1985 May 30
- [The effect of alpha-tocopherol in a wide range of concentrations on the structural features of the lipid bilayer regions of endoplasmic reticulum membranes in the mouse liver cells in vitro].
- Authors: Belov VV, Mal'tseva EL, Pal'mina NP
- Issue date: 2003 May-Jun