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dc.contributor.authorTugwood, Jonathan D
dc.contributor.authorLau, W H
dc.contributor.authorO, S K
dc.contributor.authorTsao, S Y
dc.contributor.authorMartin, W M
dc.contributor.authorShiu, W
dc.contributor.authorDesgranges, C
dc.contributor.authorJones, Philip H
dc.contributor.authorArrand, John R
dc.date.accessioned2010-10-20T11:03:07Z
dc.date.available2010-10-20T11:03:07Z
dc.date.issued1987-04
dc.identifier.citationEpstein-Barr virus-specific transcription in normal and malignant nasopharyngeal biopsies and in lymphocytes from healthy donors and infectious mononucleosis patients. 1987, 68 ( Pt 4):1081-91 J. Gen. Virol.en
dc.identifier.issn0022-1317
dc.identifier.pmid3033127
dc.identifier.doi10.1099/0022-1317-68-4-1081
dc.identifier.urihttp://hdl.handle.net/10541/113545
dc.description.abstractCytoplasmic RNA was prepared from biopsy material obtained from the nasopharynx of normal individuals and nasopharyngeal carcinoma (NPC) patients. Similar RNA preparations were prepared from lymphocytes of healthy donors and infectious mononucleosis patients. RNA was radioactively labelled in vitro and hybridized to cloned fragments of B95-8 Epstein-Barr virus (EBV). In all seropositive cases the minimum pattern of EBV-specific RNA expression was like that observed previously in latently infected, EBV-positive lymphoblastoid cell lines or Burkitt's lymphoma biopsies. A novel observation was that all of the control biopsies from normal nasopharynxes expressed EBV-specific RNA, some of which appeared to be associated with a more active state of EBV infection. The pattern of expression in NPC patients was similar to that of the normal donors but showed some variations in complexity. In general the virus-specified small RNAs were not present in normal nasopharyngeal tissue but were expressed in the NPC biopsies.
dc.language.isoenen
dc.subjectNasopharyngeal Canceren
dc.subject.meshBiopsy
dc.subject.meshCarcinoma
dc.subject.meshGenes, Viral
dc.subject.meshHerpesvirus 4, Human
dc.subject.meshHumans
dc.subject.meshInfectious Mononucleosis
dc.subject.meshLymphocytes
dc.subject.meshNasopharyngeal Neoplasms
dc.subject.meshNasopharynx
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshRNA, Viral
dc.subject.meshTranscription, Genetic
dc.titleEpstein-Barr virus-specific transcription in normal and malignant nasopharyngeal biopsies and in lymphocytes from healthy donors and infectious mononucleosis patients.en
dc.typeArticleen
dc.identifier.journalJournal of General Virologyen
html.description.abstractCytoplasmic RNA was prepared from biopsy material obtained from the nasopharynx of normal individuals and nasopharyngeal carcinoma (NPC) patients. Similar RNA preparations were prepared from lymphocytes of healthy donors and infectious mononucleosis patients. RNA was radioactively labelled in vitro and hybridized to cloned fragments of B95-8 Epstein-Barr virus (EBV). In all seropositive cases the minimum pattern of EBV-specific RNA expression was like that observed previously in latently infected, EBV-positive lymphoblastoid cell lines or Burkitt's lymphoma biopsies. A novel observation was that all of the control biopsies from normal nasopharynxes expressed EBV-specific RNA, some of which appeared to be associated with a more active state of EBV infection. The pattern of expression in NPC patients was similar to that of the normal donors but showed some variations in complexity. In general the virus-specified small RNAs were not present in normal nasopharyngeal tissue but were expressed in the NPC biopsies.


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