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dc.contributor.authorDodd, Nicholas J F
dc.contributor.authorKumar, Shant
dc.date.accessioned2010-10-20T11:20:43Z
dc.date.available2010-10-20T11:20:43Z
dc.date.issued1987
dc.identifier.citationAn ESR spin label study of childhood kidney tumours., 7 (4B):761-4 Anticancer Res.en
dc.identifier.issn0250-7005
dc.identifier.pmid2823683
dc.identifier.urihttp://hdl.handle.net/10541/113522
dc.description.abstractThe isolated membranes of cells from normal kidneys, foetal kidneys, Wilms' tumours, and bone-metastasizing renal tumours of childhood (BMRTC) have been examined by electron spin resonance, using the lipophilic spin label 5-doxyl stearic acid. No differences in membrane rigidity were detected between normal kidney cells and Wilms' tumour cells, over the temperature range 5 to 40 degrees C. The membranes of foetal kidney cells showed no significant difference at physiological temperatures, but were more rigid than the membranes of normal kidney cells at lower temperatures. In contrast, BMRTC cells showed lower membrane rigidity than normal kidney cells at all temperatures studied, BMRTC cells, unlike normal kidney and Wilms' tumour cells, lack surface fibronectin, but addition of fibronectin to BMRTC cell membranes, in suspension, had no detectable effect on their rigidity.
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectWilms Tumouren
dc.subjectKidney Canceren
dc.subject.meshCell Membrane
dc.subject.meshCells, Cultured
dc.subject.meshElectron Spin Resonance Spectroscopy
dc.subject.meshFibronectins
dc.subject.meshHumans
dc.subject.meshKidney
dc.subject.meshKidney Neoplasms
dc.subject.meshMembrane Fluidity
dc.subject.meshSpin Labels
dc.subject.meshTemperature
dc.subject.meshTumor Cells, Cultured
dc.subject.meshWilms Tumor
dc.titleAn ESR spin label study of childhood kidney tumours.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.en
dc.identifier.journalAnticancer Researchen
html.description.abstractThe isolated membranes of cells from normal kidneys, foetal kidneys, Wilms' tumours, and bone-metastasizing renal tumours of childhood (BMRTC) have been examined by electron spin resonance, using the lipophilic spin label 5-doxyl stearic acid. No differences in membrane rigidity were detected between normal kidney cells and Wilms' tumour cells, over the temperature range 5 to 40 degrees C. The membranes of foetal kidney cells showed no significant difference at physiological temperatures, but were more rigid than the membranes of normal kidney cells at lower temperatures. In contrast, BMRTC cells showed lower membrane rigidity than normal kidney cells at all temperatures studied, BMRTC cells, unlike normal kidney and Wilms' tumour cells, lack surface fibronectin, but addition of fibronectin to BMRTC cell membranes, in suspension, had no detectable effect on their rigidity.


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