Detection of ras gene alterations and ras proteins in colorectal cancer.
Affiliation
Department of Surgery, Christie Hospital and Holt Radium Institute, Manchester, England.Issue Date
1989-08
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Show full item recordAbstract
DNA extracted from 31 primary colorectal carcinomas was analyzed for the presence of ras gene amplification and mutations. Nine carcinomas had Ha-ras amplification and seven Ki-ras amplification. Nine carcinomas had codon 12 Ki-ras mutations. Immunohistochemical staining for ras proteins revealed a normal membrane association in normal mucosa and benign polyps but an abnormal cytoplasmic distribution in carcinomas. Amplification, mutations, and immunohistochemical staining were independent of histologic differentiation, Dukes' stage, or DNA ploidy status. This study demonstrates that abnormalities of ras genes are a common finding in colorectal carcinomas. They are potentially important biologic changes associated with malignancy, although they do not appear to be related to clinical behavior.Citation
Detection of ras gene alterations and ras proteins in colorectal cancer. 1989, 32 (8):659-64 Dis. Colon RectumJournal
Diseases of the Colon and RectumDOI
10.1007/BF02555769PubMed ID
2666051Type
ArticleLanguage
enISSN
0012-3706ae974a485f413a2113503eed53cd6c53
10.1007/BF02555769
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