Methods to assess changes in the pattern of nuclear phosphoinositides.
Authors
Divecha, NullinAffiliation
CRUK Inositide Laboratory, Paterson Institute for Cancer Research, Manchester, UK. ndivecha@picr.man.ac.ukIssue Date
2010
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Phosphatidylinositol (PtdIns) and its phosphorylated derivatives represent less than 5% of total membrane phospholipids in cells. Despite their low abundance, they form a dynamic signalling system that is regulated in response to a variety of extra and intra-cellular cues (Curr Opin Genet Dev 14:196-202, 2004). Phosphoinositides and the enzymes that synthesize them are found in many different sub-cellular compartments including the nuclear matrix, heterochromatin, and sites of active RNA splicing, suggesting that phosphoinositides may regulate specific functions within the nuclear compartment (Nat Rev Mol Cell Biol 4:349-360, 2003; Curr Top Microbiol Immunol 282:177-206, 2004; Cell Mol Life Sci 61:1143-1156, 2004). The existence of distinct sub-cellular pools has led to the challenging task of understanding how the different pools are regulated and how changes in the mass of lipids within the nucleus can modulate nuclear specific pathways. Here we describe methods to determine how enzymatic activities that modulate nuclear phosphoinositides are changed in response to extracellular stimuli.Citation
Methods to assess changes in the pattern of nuclear phosphoinositides. 2010, 645:165-77 Methods Mol BiolJournal
Methods in Molecular BiologyDOI
10.1007/978-1-60327-175-2_11PubMed ID
20645188Type
ArticleLanguage
enISSN
1940-60291064-3745
ae974a485f413a2113503eed53cd6c53
10.1007/978-1-60327-175-2_11
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