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    Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus.

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    Authors
    Stewart, James P
    Hampson, Ian N
    Heinrich, Hans-Werner
    Mackett, Mike
    Arrand, John R
    Affiliation
    Paterson Institute for Cancer Research, Manchester, U.K.
    Issue Date
    1989-05
    
    Metadata
    Show full item record
    Abstract
    The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.
    Citation
    Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus. 1989, 70 ( Pt 5):1231-7 J. Gen. Virol.
    Journal
    The Journal of General Virology
    URI
    http://hdl.handle.net/10541/111021
    DOI
    10.1099/0022-1317-70-5-1231
    PubMed ID
    2543776
    Type
    Article
    Language
    en
    ISSN
    0022-1317
    ae974a485f413a2113503eed53cd6c53
    10.1099/0022-1317-70-5-1231
    Scopus Count
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    All Paterson Institute for Cancer Research

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