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    The translaminal fibrils of the human amnion basement membrane.

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    Authors
    Campbell, S
    Allen, Terence D
    Moser, B B
    Aplin, J D
    Affiliation
    Department of Medical Microbiology, University of Manchester, UK.
    Issue Date
    1989-10
    
    Metadata
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    Abstract
    The organisation of extracellular matrix beneath the human amniotic epithelium was investigated in order that the co-ordinate synthesis of basal lamina and stroma by these cells could be better understood. Transmission electron microscopy of intact tissue confirmed that stromal matrix fibrils are located between the cell surface and the basal lamina, and also penetrate the lamina. The distribution of the supralaminal fibrils and their association with the lamina was further investigated by scanning electron microscopy (SEM) after removal of the overlying epithelium. Five complementary procedures were used to remove the cells from the underlying lamina. Trypsin-EDTA treatment caused the epithelial cells to retract or detach from the lamina. SDS or ammonium hydroxide was used to extract the epithelium, which was then removed by physical shearing. Transmission electron microscopy (TEM) confirmed that the lamina densa and supralaminal fibres were present after extraction by these agents. Incubation in CHAPS, a zwiterionic detergent, did not remove the epithelium but permitted exposure of the basal lamina by mechanical scoring. Extraction with boric acid followed by osmium tetroxide produced epithelial disruption and revealed the lamina and stroma in different areas. Although the extraction pattern was different in each case, all of the five methods confirmed that individual fibrils and fibril bundles are present on the apical surface of, and enter, the lamina densa. Examination of the stromal surface of the basal lamina after fracture revealed fibrils passing from the stroma into the lamina densa. We therefore suggest that, in this tissue, newly synthesised stromal matrix components appear in an assembled fibrillar form between the basal cell surface and the basal lamina before becoming associated with the sublaminal stroma.
    Citation
    The translaminal fibrils of the human amnion basement membrane. 1989, 94 ( Pt 2):307-18 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/111009
    PubMed ID
    2621227
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

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