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    Crypt base columnar cells in ileum of BDF1 male mice--their numbers and some features of their proliferation.

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    Authors
    Chwalinski, S
    Potten, Christopher S
    Affiliation
    Department of Epithelial Biology, Christie Hospital and Holt Radium Institute, Manchester, England.
    Issue Date
    1989-12
    
    Metadata
    Show full item record
    Abstract
    Some features of the proliferative cells at the bottom of the ileal crypts in BDF1 mice have been studied in relation to the distribution of Paneth cells (PC) in an attempt to clarify the nature and function of these crypt base columnar cells (BCC) and to elucidate some aspects of the role of the microenvironment created by the PC. Longitudinal sections of crypts have shown that the ratio of PC to the BCC, which are scattered amongst the PC, is 2.7:1 in sections or approximately 29 PC and 9 BCC per whole crypt, i.e., a ratio of 3.2:1. The labelling index of BCC is about 35%, which is comparable to that of mid-crypt columnar cells. Although the BCC do become labeled, it is concluded that they cannot create vertical pairs or runs of several adjacent BCC since this would seriously disturb the distribution of Paneth cells. Only in dividing crypts are such runs (consisting of 3 to 5 cells) observed. The ability of BCC to synthesize DNA is not dependent on their position in the Paneth cell zone. In 95% of the crypts, the highest Paneth cell is below the 7th cell position from the bottom of the crypt, and the positions of the highest PC on either side of a given crypt are similar. The secreted granules or the cytoplasm of PC specifically bind pokeweed lectin, and this can be used for identification. Tracer doses of 3HTdR (37 kBq/gm body weight) result in the histological death of some BCC, and these damaged cells are evenly distributed throughout the Paneth cell zone. These tracer doses are somewhat selectively incorporated into BCC, i.e., the BCC have a higher grain count in autoradiographs, probably because they possess more thymidine kinase enzyme activity. This ability is very sensitive to the withdrawal of food, because 24 hr of fasting abolished the observed gradient in the intensity of labelling, which is very well correlated with the distribution of BCC. Regeneration of the crypts following cytotoxic exposure to Ara-C is initiated at the base of the crypt and hence may involve the BCC with possible help from the Paneth cells. The latter are insensitive to cytotoxic (S phase specific) agents and may help in the regeneration by preserving the architecture of the base of the crypt.
    Citation
    Crypt base columnar cells in ileum of BDF1 male mice--their numbers and some features of their proliferation. 1989, 186 (4):397-406 Am. J. Anat.
    Journal
    The American Journal of Anatomy
    URI
    http://hdl.handle.net/10541/111002
    DOI
    10.1002/aja.1001860409
    PubMed ID
    2589224
    Type
    Article
    Language
    en
    ISSN
    0002-9106
    ae974a485f413a2113503eed53cd6c53
    10.1002/aja.1001860409
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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