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dc.contributor.authorFan, Chun-Yang
dc.contributor.authorButler, W H
dc.contributor.authorO'Connor, Peter J
dc.date.accessioned2010-09-10T13:37:28Z
dc.date.available2010-09-10T13:37:28Z
dc.date.issued1989-10
dc.identifier.citationCell and tissue specific localization of O6-methylguanine in the DNA of rats given N-nitrosodimethylamine: effects of protein deficient and normal diets. 1989, 10 (10):1967-70 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid2507193
dc.identifier.doi10.1093/carcin/10.10.1967
dc.identifier.urihttp://hdl.handle.net/10541/110997
dc.description.abstractAn immunohistochemical procedure which is specific for the detection of O6-methylguanine (O6-MeG) in nuclear DNA has been used to examine tissues of rats after treatment with N-nitrosodimethylamine. In liver, the principal cells that are positive for O6-MeG are the centrilobular hepatocytes and the sinusoidal cells. In the kidney cortex the proximal tubules, the mesenchymal elements and the capsules of Bowman are positive, whilst the cells of the glomeruli and the renal medulla are negative. Maintaining the animals on a protein-free diet prior to treatment with the nitrosamine significantly reduces the width of the band of positive cells around the central vein in the liver and increases the number and intensity of positively staining cells in the renal cortex. Subject to subsequent modification by DNA repair and DNA replication, the procedure therefore identifies cells which are potential targets for nitrosamine induced toxicity and carcinogenicity in liver and kidney.
dc.language.isoenen
dc.subject.meshAlkylation
dc.subject.meshAnimals
dc.subject.meshCell Nucleus
dc.subject.meshDNA
dc.subject.meshDiethylnitrosamine
dc.subject.meshGuanine
dc.subject.meshImmunohistochemistry
dc.subject.meshKidney
dc.subject.meshLiver
dc.subject.meshMale
dc.subject.meshProtein-Energy Malnutrition
dc.subject.meshRats
dc.subject.meshRats, Inbred Strains
dc.subject.meshReference Values
dc.titleCell and tissue specific localization of O6-methylguanine in the DNA of rats given N-nitrosodimethylamine: effects of protein deficient and normal diets.en
dc.typeArticleen
dc.contributor.departmentCRC Section of Carcinogenesis, Christie Hospital and Holt Radium Institute, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
html.description.abstractAn immunohistochemical procedure which is specific for the detection of O6-methylguanine (O6-MeG) in nuclear DNA has been used to examine tissues of rats after treatment with N-nitrosodimethylamine. In liver, the principal cells that are positive for O6-MeG are the centrilobular hepatocytes and the sinusoidal cells. In the kidney cortex the proximal tubules, the mesenchymal elements and the capsules of Bowman are positive, whilst the cells of the glomeruli and the renal medulla are negative. Maintaining the animals on a protein-free diet prior to treatment with the nitrosamine significantly reduces the width of the band of positive cells around the central vein in the liver and increases the number and intensity of positively staining cells in the renal cortex. Subject to subsequent modification by DNA repair and DNA replication, the procedure therefore identifies cells which are potential targets for nitrosamine induced toxicity and carcinogenicity in liver and kidney.


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