Cell and tissue specific localization of O6-methylguanine in the DNA of rats given N-nitrosodimethylamine: effects of protein deficient and normal diets.
AffiliationCRC Section of Carcinogenesis, Christie Hospital and Holt Radium Institute, Manchester, UK.
MetadataShow full item record
AbstractAn immunohistochemical procedure which is specific for the detection of O6-methylguanine (O6-MeG) in nuclear DNA has been used to examine tissues of rats after treatment with N-nitrosodimethylamine. In liver, the principal cells that are positive for O6-MeG are the centrilobular hepatocytes and the sinusoidal cells. In the kidney cortex the proximal tubules, the mesenchymal elements and the capsules of Bowman are positive, whilst the cells of the glomeruli and the renal medulla are negative. Maintaining the animals on a protein-free diet prior to treatment with the nitrosamine significantly reduces the width of the band of positive cells around the central vein in the liver and increases the number and intensity of positively staining cells in the renal cortex. Subject to subsequent modification by DNA repair and DNA replication, the procedure therefore identifies cells which are potential targets for nitrosamine induced toxicity and carcinogenicity in liver and kidney.
CitationCell and tissue specific localization of O6-methylguanine in the DNA of rats given N-nitrosodimethylamine: effects of protein deficient and normal diets. 1989, 10 (10):1967-70 Carcinogenesis
- Immunocytochemical analysis of O6-alkylguanine shows tissue specific formation in and removal from esophageal and liver DNA in rats treated with methylbenzylnitrosamine, dimethylnitrosamine, diethylnitrosamine and ethylnitrosourea.
- Authors: Scherer E, Van Den Berg T, Vermeulen E, Winterwerp HH, Den Engelse L
- Issue date: 1989 Jul 1
- Immunocytochemical studies on the formation and repair of O6-alkylguanine in rat tissues.
- Authors: Scherer E, Jenner AA, den Engelse L
- Issue date: 1987
- Alkylation of rodent tissue DNA induced by N-nitrosobis(2-hydroxypropyl)amine.
- Authors: Kokkinakis DM
- Issue date: 1992 May
- Cell specific DNA alkylation in target and non-target organs of N-nitrosobis(2-oxopropyl)amine-induced carcinogenesis in hamster and rat.
- Authors: Bax J, Schippers-Gillissen C, Woutersen RA, Scherer E
- Issue date: 1991 Apr
- Promutagenic lesions persist in the DNA of target cells for nitrosamine-induced carcinogenesis.
- Authors: Fan CY, Butler WH, O'Connor PJ
- Issue date: 1991