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dc.contributor.authorDavidson, Susan E
dc.contributor.authorWest, Catharine M L
dc.contributor.authorRoberts, Stephen A
dc.contributor.authorHendry, Jolyon H
dc.contributor.authorHunter, Robin D
dc.date.accessioned2010-08-18T15:19:37Z
dc.date.available2010-08-18T15:19:37Z
dc.date.issued1990-08
dc.identifier.citationRadiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity. 1990, 18 (4):349-56 Radiother Oncolen
dc.identifier.issn0167-8140
dc.identifier.pmid2244021
dc.identifier.doi10.1016/0167-8140(90)90115-D
dc.identifier.urihttp://hdl.handle.net/10541/109858
dc.description.abstractBiopsies from 89 patients with cervical carcinoma were studied using a clonogenic assay to obtain values for the surviving fraction at 2 Gy (SF2). Heterogeneity in intrinsic radiosensitivity was investigated by independently processing multiple biopsies from 18 tumors. No significant differences between intra-tumour SF2 values were demonstrated (p = 0.30). The results have shown that intra-tumour heterogeneity is not a limitation to radiosensitivity testing using the Courtenay-Mills assay. A wide range of values (0.13-0.97) for SF2 was obtained with a mean value of 0.47 +/- 0.18 (+/- 1 S.D., CV = 38%) for 52 squamous cell carcinomas and 0.59 +/- 0.27 for four adenocarcinomas. There were statistically significant differences between the individual tumours (p less than 0.001). From the analysis-of-variance of all the SF2 results it appears to be the surviving fractions below about 0.40 and those above about 0.7 which show significant differences in radiosensitivity between pairs of tumours (p = 0.05). Also 36% of the values of SF2 show significant differences from the mean SF2 of all tumours. The storage of tumour cell suspensions in liquid nitrogen improved the colony-forming efficiency (CFE) but it did not alter the radiosensitivity.
dc.language.isoenen
dc.subjectTumour Stem Cell Assayen
dc.subjectUterine Cervical Canceren
dc.subject.meshAnalysis of Variance
dc.subject.meshCarcinoma
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshRadiation Tolerance
dc.subject.meshTumor Stem Cell Assay
dc.subject.meshUterine Cervical Neoplasms
dc.titleRadiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiobiology, Paterson Institute for Cancer Research, Manchester, U.K.en
dc.identifier.journalRadiotherapy and Oncologyen
html.description.abstractBiopsies from 89 patients with cervical carcinoma were studied using a clonogenic assay to obtain values for the surviving fraction at 2 Gy (SF2). Heterogeneity in intrinsic radiosensitivity was investigated by independently processing multiple biopsies from 18 tumors. No significant differences between intra-tumour SF2 values were demonstrated (p = 0.30). The results have shown that intra-tumour heterogeneity is not a limitation to radiosensitivity testing using the Courtenay-Mills assay. A wide range of values (0.13-0.97) for SF2 was obtained with a mean value of 0.47 +/- 0.18 (+/- 1 S.D., CV = 38%) for 52 squamous cell carcinomas and 0.59 +/- 0.27 for four adenocarcinomas. There were statistically significant differences between the individual tumours (p less than 0.001). From the analysis-of-variance of all the SF2 results it appears to be the surviving fractions below about 0.40 and those above about 0.7 which show significant differences in radiosensitivity between pairs of tumours (p = 0.05). Also 36% of the values of SF2 show significant differences from the mean SF2 of all tumours. The storage of tumour cell suspensions in liquid nitrogen improved the colony-forming efficiency (CFE) but it did not alter the radiosensitivity.


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