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dc.contributor.authorWest, Catharine M Len
dc.contributor.authorWest, David Cen
dc.contributor.authorKumar, Shanten
dc.contributor.authorMoore, James Ven
dc.date.accessioned2010-08-18T13:25:24Z
dc.date.available2010-08-18T13:25:24Z
dc.date.issued1990-07
dc.identifier.citationA comparison of the sensitivity to photodynamic treatment of endothelial and tumour cells in different proliferative states. 1990, 58 (1):145-56 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002
dc.identifier.pmid1973432
dc.identifier.doi10.1080/09553009014551501
dc.identifier.urihttp://hdl.handle.net/10541/109854
dc.description.abstractBovine aorta endothelial and human colon adenocarcinoma (WiDr) cells were exposed to haematoporphyrin derivative plus light. Cell survival was determined using a [3H]thymidine incorporation and a clonogenic assay for both cell types. The endothelial cells were more sensitive to photodynamic treatment. This could be explained in terms of increased drug uptake into the endothelial cells despite there being no difference in cell volume between the cells. For both tumour and endothelial cells, exponentially growing cells were more sensitive to photodynamic treatment than plateau-phase cells. This was associated with a reduction in drug uptake into plateau-phase endothelial cells. However, there was no difference in uptake into exponentially growing and plateau-phase WiDr cells.
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectHaematoporphyrin Photoradiationen
dc.subject.meshAnimals
dc.subject.meshCattle
dc.subject.meshCell Cycle
dc.subject.meshCell Line
dc.subject.meshEndothelium, Vascular
dc.subject.meshHematoporphyrin Photoradiation
dc.subject.meshHumans
dc.subject.meshPhotochemotherapy
dc.subject.meshTumor Cells, Cultured
dc.titleA comparison of the sensitivity to photodynamic treatment of endothelial and tumour cells in different proliferative states.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Radiobiology, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalInternational Journal of Radiation Biologyen
html.description.abstractBovine aorta endothelial and human colon adenocarcinoma (WiDr) cells were exposed to haematoporphyrin derivative plus light. Cell survival was determined using a [3H]thymidine incorporation and a clonogenic assay for both cell types. The endothelial cells were more sensitive to photodynamic treatment. This could be explained in terms of increased drug uptake into the endothelial cells despite there being no difference in cell volume between the cells. For both tumour and endothelial cells, exponentially growing cells were more sensitive to photodynamic treatment than plateau-phase cells. This was associated with a reduction in drug uptake into plateau-phase endothelial cells. However, there was no difference in uptake into exponentially growing and plateau-phase WiDr cells.


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