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    Immunological response of nasopharyngeal carcinoma patients to the Epstein-Barr-virus-coded thymidine kinase expressed in Escherichia coli.

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    Authors
    Littler, Edward
    Newman, William G
    Arrand, John R
    Affiliation
    Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
    Issue Date
    1990-06-15
    
    Metadata
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    Abstract
    A bacterial expression system which produces large amounts of the Epstein-Barr-virus-coded thymidine kinase has been developed and used to produce protein for Western blot analysis of a number of human antisera. Interestingly, only sera from nasopharyngeal carcinoma (NPC) patients had any detectable IgA antibody which reacted with the EBV TK. These findings provided the basis for ELISA tests using a crude lysate of the E. coli cells expressing the EBV TK as target antigen. Sera from NPC patients showed high levels of IgA reactive antibodies in this test while other sera did not.
    Citation
    Immunological response of nasopharyngeal carcinoma patients to the Epstein-Barr-virus-coded thymidine kinase expressed in Escherichia coli. 1990, 45 (6):1028-32 Int. J. Cancer
    Journal
    International Journal of Cancer.
    URI
    http://hdl.handle.net/10541/109849
    DOI
    10.1002/ijc.2910450608
    PubMed ID
    2161795
    Type
    Article
    Language
    en
    ISSN
    0020-7136
    ae974a485f413a2113503eed53cd6c53
    10.1002/ijc.2910450608
    Scopus Count
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    All Paterson Institute for Cancer Research

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