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dc.contributor.authorButler, Johnen
dc.contributor.authorDzielendziak, Aen
dc.contributor.authorLea, J Sen
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorHoey, Brigid Men
dc.date.accessioned2010-08-18T12:56:44Z
dc.date.available2010-08-18T12:56:44Z
dc.date.issued1990
dc.identifier.citationContrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones. 1990, 8 (4-6):231-9 Free Radic. Res. Commun.en
dc.identifier.issn8755-0199
dc.identifier.pmid2162311
dc.identifier.doi10.3109/10715769009053356
dc.identifier.urihttp://hdl.handle.net/10541/109845
dc.description.abstractThe mechanisms of cytotoxicity of the antitumour diaziridinylbenzoquinones, AZQ and BZQ, have been investigated. HPLC analysis has been used to study the products as well as the rate of decomposition of acid-assisted ring-opening in aqueous medium as a function of pH. Microconcentrators with a molecular weight cutoff of 30 kDa were utilised to study the covalent binding of both compounds to calf thymus DNA. Radical production of both compounds in K562 cell incubations was followed using ESR and their uptake into K562 cells was monitored using radiolabelled compounds. The results show that these two diaziridinylbenzoquinones, although very similar in structure, have diverse mechanisms of cytotoxicity. The implications of these findings are discussed in the light of antitumor action.
dc.language.isoenen
dc.subjectAnticancerous Agentsen
dc.subject.meshAlkylating Agents
dc.subject.meshAnimals
dc.subject.meshAntineoplastic Agents
dc.subject.meshAziridines
dc.subject.meshBenzoquinones
dc.subject.meshCell Survival
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshDNA
dc.subject.meshDrug Stability
dc.subject.meshElectron Spin Resonance Spectroscopy
dc.subject.meshFree Radicals
dc.subject.meshHumans
dc.subject.meshHydrogen-Ion Concentration
dc.subject.meshKinetics
dc.subject.meshOxidation-Reduction
dc.titleContrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biophysical Chemistry, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.en
dc.identifier.journalFree Radical Research Communicationsen
html.description.abstractThe mechanisms of cytotoxicity of the antitumour diaziridinylbenzoquinones, AZQ and BZQ, have been investigated. HPLC analysis has been used to study the products as well as the rate of decomposition of acid-assisted ring-opening in aqueous medium as a function of pH. Microconcentrators with a molecular weight cutoff of 30 kDa were utilised to study the covalent binding of both compounds to calf thymus DNA. Radical production of both compounds in K562 cell incubations was followed using ESR and their uptake into K562 cells was monitored using radiolabelled compounds. The results show that these two diaziridinylbenzoquinones, although very similar in structure, have diverse mechanisms of cytotoxicity. The implications of these findings are discussed in the light of antitumor action.


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