Copper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure.
Affiliation
Paterson Institute for Cancer Research, Department of Carcinogenesis, Withington, Manchester, UK.Issue Date
1990-11
Metadata
Show full item recordAbstract
Copper phthalocyanine tetrasulphonic acid (CPTS) functions were introduced into magnetic semi-permeable polyethyleneimine (PEI) microcapsules in order to create a recoverable scavenging system for trapping and biomonitoring, within the gastrointestinal cavity, of mutagens having a planar molecular structure. Stable ionic CPTS and covalent (thionylated CPTS, TCPTS) adducts to the microcapsule PEI were produced and shown to trap benzo[a]pyrene (B[a]P) in vitro in relation to the porphyrin/B[a]P ratio employed. 3-hydroxy B[a]P and B[a]P 3,6-dione from a crude B[a]P metabolite mixture, and a set of planar mutagens from crude opium/morphine pyrolysate mixtures could also be recovered in 7-86% yields after shaking with modified microcapsules followed by methanol/ammonia (50:1) desorption. Tetraols derived from B[a]P 7,8-diol-9,10 epoxide could also be recovered. Modified microcapsules were recovered magnetically from faeces of rats treated with [14C]B[a]P, and 45-51% of trapped radioactivity could be directly desorbed for HPLC assay compared with 30% for unmodified microscapsules. The relative extent of trapping by unmodified or CPTS- or TCPTS-modified microcapsules was different for various substrates, and it appears that the copper phthalocyanine tetrasulphonic acid moiety competes with another unidentified absorption/desorption structure in the microcapsules. These results show that selective and reversible trapping of carcinogens/mutagens having planar molecular structure can be achieved within the gastrointestinal tract.Citation
Copper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure. 1990, 11 (11):1989-93 CarcinogenesisJournal
CarcinogenesisDOI
10.1093/carcin/11.11.1989PubMed ID
2225331Type
ArticleLanguage
enISSN
0143-3334ae974a485f413a2113503eed53cd6c53
10.1093/carcin/11.11.1989
Scopus Count
Collections
Related articles
- Modulating effects in human diets of dietary fibre and beef, and of time and dose on the reactive microcapsule trapping of benzo[a]pyrene metabolites in the rat gastrointestinal tract.
- Authors: O'Neill IK, Bingham S, Povey AC, Brouet I, Bereziat JC
- Issue date: 1990 Apr
- Systematic modulation by human diet levels of dietary fibre and beef on metabolism and disposition of benzo[a]pyrene in the gastrointestinal tract of Fischer F344 rats.
- Authors: O'Neill IK, Povey AC, Bingham S, Cardis E
- Issue date: 1990 Apr
- Entrapment by magnetic microcapsules of the protein pyrolysates IQ, PhIP and Glu-P-1, and alteration of IQ metabolite exposure within the rat gastrointestinal tract by risk-modulating components of the human diet.
- Authors: O'Neill I, Ohgaki H, Ellul A, Turesky RJ
- Issue date: 1992 Dec
- Binding of benzo[a]pyrene metabolites in the rat intestinal lumen by magnetic polyethyleneimine microcapsules following an intragastric dose of [14C]benzo[a]pyrene.
- Authors: Povey AC, Brouet I, Bartsch H, O'Neill IK
- Issue date: 1987 Jun
- Trapping of chemical carcinogens with magnetic polyethyleneimine microcapsules: III. In vivo trapping of electrophiles from N-methyl-N-nitrosourea and recovery from feces.
- Authors: Povey AC, Brouet I, Nixon JR, O'Neill IK
- Issue date: 1987 Mar