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    Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.

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    Authors
    Rossiter, Belinda J
    Muzny, D M
    Hampson, Ian N
    Caskey, C T
    Fox, Margaret
    Affiliation
    Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.
    Issue Date
    1990-11
    
    Metadata
    Show full item record
    Abstract
    The Chinese hamster hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient cell line TG15 produces apparently normal HPRT mRNA by northern analysis and was therefore presumed to contain a point mutation within the coding region. Sequencing cDNA from the TG15 cell line revealed an A to G transition which results in the substitution of the amino acid glycine for aspartic acid at position 135. TG15 cells revert to wild-type HPRT activity upon exposure to monofunctional alkylating agents. A rapid test to assay the site of the TG15 point mutation has been developed, utilizing the polymerase chain reaction and allele-specific oligonucleotide screening. In all revertants studied, the original point mutation has been corrected to the wild-type sequence. The TG15 point mutation lies within a proposed catalytic domain of the HPRT protein in common with other phosphoribosyltransferases.
    Citation
    Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence. 1990, 5 (6):605-8 Mutagenesis
    Journal
    Mutagenesis
    URI
    http://hdl.handle.net/10541/109815
    DOI
    10.1093/mutage/5.6.605
    PubMed ID
    1702179
    Type
    Article
    Language
    en
    ISSN
    0267-8357
    ae974a485f413a2113503eed53cd6c53
    10.1093/mutage/5.6.605
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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