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    Pericytes derived from the retinal microvasculature undergo calcification in vitro.

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    Authors
    Schor, Ana M
    Allen, Terence D
    Canfield, Ann E
    Sloan, P
    Schor, Seth L
    Affiliation
    CRC Department of Medical Oncology, Manchester University, Christie Hospital & Holt Radium Institute, UK.
    Issue Date
    1990-11
    
    Metadata
    Show full item record
    Abstract
    Pericytes isolated from the bovine retinal microvasculature retain characteristic features of their in vivo counterparts, such as the presence of glycogen deposits, long filamentous processes, prominent microfilament bundles and the ability to display two distinct and reversible phenotypes. Time-lapse video-microscopy demonstrated that pericytes tend to overlap and aggregate, even in sparse cultures. After reaching confluence, they form multilayered areas that retract away from each other, resulting in the formation of multicellular nodules. These nodules increase in size and cellularity by going through repeated 5- to 6-h cycles of anchoring, spreading, cell proliferation and retraction. Alkaline phosphatase was not detected in pericytes at subconfluent or confluent densities, but this enzyme was expressed in areas of high cell density, such as multilayers and nodules. Pericytes synthesise and deposit an extracellular matrix at all stages of their in vitro development, including nodule formation. The matrix within the nodules contains cross-striated collagen fibres and matrix vesicles. Needle-like crystals of hydroxyapatite appear to be deposited within the matrix, thus leading to massive calcification of the nodule. Calcification, as assessed by electron microscopy, histochemical staining and X-ray microprobe analysis, occurred on plastic and collagen substrate in the absence of disodium-beta-glycerophosphate. The addition of this compound at 5 or 10 mM or the use of a collagen substratum (rather than plastic), brought forward the process of nodule formation and calcification by 3-6 days. Our results suggest that retinal pericytes may differentiate in vitro along the osteogenic pathway.
    Citation
    Pericytes derived from the retinal microvasculature undergo calcification in vitro. 1990, 97 ( Pt 3):449-61 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/109814
    PubMed ID
    2074265
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

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