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Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.

dc.contributor.authorLittler, Edward
dc.contributor.authorLawrence, G
dc.contributor.authorLiu, Mei-Ying
dc.contributor.authorBarrell, B G
dc.contributor.authorArrand, John R
dc.date.accessioned2010-08-18T08:23:55Z
dc.date.available2010-08-18T08:23:55Z
dc.date.issued1990-02
dc.identifier.citationIdentification, cloning, and expression of the major capsid protein gene of human herpesvirus 6. 1990, 64 (2):714-22 J. Virol.en
dc.identifier.issn0022-538X
dc.identifier.pmid2153237
dc.identifier.urihttp://hdl.handle.net/10541/109813
dc.description.abstractDNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshBase Sequence
dc.subject.meshCapsid
dc.subject.meshCells, Cultured
dc.subject.meshCloning, Molecular
dc.subject.meshDNA, Viral
dc.subject.meshFetal Blood
dc.subject.meshGene Expression
dc.subject.meshGenes, Viral
dc.subject.meshHumans
dc.subject.meshLymphocytes
dc.subject.meshMolecular Sequence Data
dc.subject.meshPlasmids
dc.subject.meshRestriction Mapping
dc.subject.meshSequence Homology, Nucleic Acid
dc.subject.meshSimplexvirus
dc.subject.meshViral Structural Proteins
dc.titleIdentification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biology, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.en
dc.identifier.journalJournal of Virologyen
html.description.abstractDNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.


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